骨髓间充质干细胞对冈田酸致神经细胞损伤的修复作用

发布时间：2018-06-09 11:54

本文选题：骨髓间充质干细胞 + 冈田酸　；参考：《解放军医学杂志》2017年05期

【摘要】：目的观察人骨髓间充质干细胞对冈田酸(OA)损伤的神经细胞是否具有修复作用。方法应用冈田酸损伤神经母细胞瘤细胞系SH-SY5Y细胞,建立阿尔茨海默病体外模型。将细胞分成正常组、损伤组和治疗组,损伤组用20nmol/L冈田酸损伤24h,治疗组在损伤24h后加入骨髓间充质干细胞的条件培养基治疗24h。采用CCK-8法检测各组细胞活力,免疫荧光染色微管微丝测定细胞树突长度和荧光面积,Western blotting检测磷酸化Tau蛋白和总Tau蛋白含量。结果冈田酸能损伤SH-SY5Y细胞,使其胞体皱缩、塌陷,出现空泡,树突缩短、断裂,微管微丝排列紊乱,而骨髓间充质干细胞条件培养基可使SH-SY5Y细胞胞体变得圆润,树突重新恢复变长,微管、微丝致密规则,荧光变强;并且能有效降低冈田酸诱导的Tau蛋白过度磷酸化水平。结论骨髓间充质干细胞对冈田酸损伤的神经细胞具有明显的修复作用。
[Abstract]:Objective to investigate the repair effect of human bone marrow mesenchymal stem cells (BMSCs) on cultured neurons injured by OAA. Methods the model of Alzheimer's disease was established by using Okadaic acid to injure neuroblastoma cell line SH-SY5Y in vitro. The cells were divided into normal group, injury group and treatment group. The injury group was treated with 20nmol / L okadaic acid for 24 h, and the treatment group was treated with the condition medium of bone marrow mesenchymal stem cells (BMSCs) for 24 h after 24 hours of injury. The cell viability was detected by CCK-8 method, and the contents of phosphorylated Tau protein and total Tau protein were detected by immunofluorescence staining microtubule microfilaments and the length of dendritic cells and fluorescence area by Western blotting. Results okadaic acid could damage SH-SY5Y cells, make their cell bodies shrink, collapse, vacuole, dendritic shortening, break, microtubule microfilament arrangement disorder, and bone marrow mesenchymal stem cell conditioned medium can make SH-SY5Y cells cell body become round. The dendrites recovered and became longer, microtubules and microfilaments became dense, fluorescence became stronger, and the over-phosphorylation level of Tau protein induced by okadaic acid was effectively reduced. Conclusion Bone marrow mesenchymal stem cells can repair the nerve cells damaged by okadaic acid.
【作者单位】：南方医科大学检验与生物技术学院;军事医学科学院野战输血研究所干细胞与再生医学研究室;
【基金】：国家高技术研究发展计划(863计划)项目(2013AA020107) 广州市健康医疗协同创新重大专项(201604020009) 广东省重大科技专项(2013A022100005) 广东省前沿与关键技术创新专项(2014B020228001,2015B020226001)~~
【分类号】：R749.16

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