吗啡精神依赖大鼠VTA-NAc-PFC神经环路NR1表达的变化

发布时间：2018-06-10 06:43

本文选题：吗啡 + 精神依赖　；参考：《神经解剖学杂志》2014年06期

【摘要】：目的:研究吗啡诱导条件性位置偏爱(conditioned place preference,CPP)大鼠中脑腹侧被盖区-伏核-前额叶皮质(VTA-NAc-PFC)神经环路各核团中N-甲基-D-天冬氨酸受体NR1亚基(NR1)表达的变化。方法:20只大鼠随机分为生理盐水对照组和吗啡条件性位置偏爱模型组,模型组采用大鼠颈背部皮下吗啡剂量递增注射(起始剂量10 mg/kg,每天递增10 mg/kg,至注射10 d时为100 mg/kg),CPP训练10 d,末次训练后48 h CPP检测确认模型建立成功后取材,利用Western Blot方法检测VTA,NAc和PFC内NR1蛋白的表达情况。结果:经CCP检测,模型组大鼠在白箱(吗啡伴药箱)停留时间在训练前和训练后分别为408±93 s和528±81 s,与生理盐水组比较(训练前和训练后分别为393±81 s和416±58 s)明显有所延长(P0.05)。Western Blot检测到吗啡诱导条件性位置偏爱大鼠的NAc核团NR1表达水平较生理盐水组显著增加(P0.05),而VTA和PFC两个核团的NR1表达水平则未见明显改变。结论:NR1在吗啡精神依赖过程中NAc核团中表达增加,而在VTA和PFC核团中没有增加。
[Abstract]:Aim: to study the expression of N- methyl-Daspartic acid receptor NR1 subunit in the ventral tegmental-nucleus accumbens and prefrontal cortex of morphine-induced conditioned place preferencein rats. Methods Twenty rats were randomly divided into normal saline control group and morphine conditioned place preference model group. Rats in the model group were given subcutaneous morphine injection (initial dose 10 mg / kg, 10 mg / kg daily, 100 mg / kg CPP training 10 days after injection, 48 h after the last training). The expression of NR1 protein in VTA-NAc and PFC was detected by Western blot. Results: by CCP, The duration of staying in white box (morphine with medicine box) in the model group was 408 卤93 s and 528 卤81 s before and after training, which was significantly longer than that in the saline group (393 卤81 s and 416 卤58 s before and after training). The expression level of NR1 in NAC nucleus of morphine induced conditioned place preference rats was significantly higher than that in normal saline group, but the expression level of NR1 in VTA and PFC was not significantly changed. Conclusion the expression of NR1 was increased in the NAC nuclei during morphine dependence, but not in the VTA and PFC nuclei.
【作者单位】：遵义医学院细胞生物学与遗传学教研室;
【基金】：贵州省2013年度联合基金计划项目;黔科合J字LKZ[2013]22号贵州省科技厅社会攻关项目,黔科合SY字[2013]3065号贵州省科技厅中药攻关项目,黔科合ZY字[2013]3002号
【分类号】：R749.61

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