二苯乙烯苷对血管性痴呆大鼠室管膜细胞增殖的影响

发布时间：2018-06-11 13:14

本文选题：二苯乙烯苷 + 血管性痴呆　；参考：《中国老年学杂志》2017年08期

【摘要】：目的探讨二苯乙烯苷(TSG)对血管性痴呆(VD)大鼠海马血流量和脑室管膜细胞变化的影响。方法实验分为假手术组、模型组、TSG组,后两组采用4血管阻断法(4-VO法)建立VD大鼠模型。假手术组麻醉及手术过程与模型组相同,但不电凝双侧椎动脉,不阻断双侧颈总动脉。水迷宫法评价行为学。应用氢清除法检测大鼠海马血流量。用5-溴脱氧尿核苷(Brd U)免疫荧光染色,显微镜下观察室管膜下区(SVZ)干细胞增殖。结果与假手术组比较,模型组大鼠学习与记忆能力下降,表现为游全程时间延长,错误次数增加(P0.01)。手术后7 d,与模型组比较,TSG组海马血流量明显提高和脑室管膜Brd U免疫荧光染色阳性细胞明显增加(P0.01)。结论 TSG可改善VD大鼠海马血流量和促进脑室管膜细胞增殖。
[Abstract]:Objective to investigate the effects of stilbene glucoside (TSGG) on the changes of hippocampal blood flow and ependymal cells in rats with vascular dementia (VD). Methods the rats were divided into sham-operation group, model group and TSG group, the latter two groups were treated with 4-VO method. The anaesthesia and operation process in the sham operation group was the same as that in the model group, but the bilateral vertebral artery was not electrocoagulated and the bilateral common carotid artery was not blocked. Water labyrinth method was used to evaluate behavior. The hippocampal blood flow was measured by hydrogen scavenging method. 5-bromodeoxyuridine (5-bromodeoxyuridine) Brd U) immunofluorescence staining was used to observe the proliferation of SVZ) stem cells in the subependymal area under microscope. Results compared with the sham operation group, the learning and memory ability of the model group was decreased, which showed that the whole travel time was prolonged and the number of errors increased (P 0.01). 7 days after operation, compared with the model group, the hippocampal blood flow and BrdU immunofluorescence staining positive cells in the brain ependymal membrane in the TSG group were significantly increased (P 0.01). Conclusion TSG can improve hippocampal blood flow and promote the proliferation of ependymal cells in VD rats.
【作者单位】：解放军第153中心医院神经内科;重庆医科大学第一附属医院重庆市神经病学重点实验室;南京医科大学第二附属医院神经内科;
【基金】：四川省杰出青年学科带头人培养基金资助(05ZQ026-020) 四川省科技攻关项目资助(05SG1866)
【分类号】：R749.1

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