PTSD样大鼠杏仁核神经元内质网分子伴侣钙网蛋白的表达

发布时间：2018-06-14 08:02

本文选题：创伤后应激障碍 + 杏仁核　；参考：《中国医科大学学报》2014年11期

【摘要】：目的检测创伤后应激障碍(PTSD)大鼠杏仁核内质网分子伴侣钙网蛋白(CRT)表达的变化。方法建立无连续单一刺激(SPS)模型,随机将大鼠分为SPS刺激后1 d组(SPS1d)、7 d组(SPS7d)、14 d组(SPS14d)及正常对照组。应用免疫组织化学、逆转录聚合酶链反应及透射电镜技术,检测各组杏仁核神经元CRT表达变化及观察神经元超微结构的改变。结果与正常对照组比较,SPS各组神经元CRT阳性表达均增加(P0.001),尤以SPS7d组最为明显。SPS各组神经元细胞核及粗面内质网均发生不同程度的改变。结论 SPS可诱导PTSD样大鼠过度内质网应激,引发杏仁核CRT表达上调并通过内质网径路发生细胞凋亡。
[Abstract]:Objective to detect the expression of CRT in rat amygdala endoplasmic reticulum (ER) molecular chaperone in post-traumatic stress disorder (PTSD) rats. Methods the model of SPS without continuous single stimulation was established. The rats were randomly divided into two groups: 1 d after SPS stimulation, 1 d after SPS stimulation and 1 d after SPS stimulation, the rats were divided into two groups: SPS group (n = 14) and normal control group (n = 14). Immunohistochemistry, reverse transcriptase polymerase chain reaction (RT-PCR) and transmission electron microscopy (TEM) were used to detect the changes of CRT expression and ultrastructure of neurons in the amygdaloid nucleus. Results compared with the normal control group, the positive expression of CRT in SPS group increased P0.001, especially in SPS7d group, the changes of nucleus and rough endoplasmic reticulum in SPS group were more obvious than those in SPS group. Conclusion SPS can induce excessive endoplasmic reticulum stress in PTSD like rats, induce the up-regulation of CRT expression in amygdala and induce apoptosis through the endoplasmic reticulum pathway.
【作者单位】：中国医科大学基础医学院电子显微学研究室;中国医科大学基础医学院组织胚胎学教研室PTSD研究室;
【基金】：国家自然科学基金(31200772) 教育部博士点基金(20132104110021) 辽宁省科学技术计划(2012225073)
【分类号】：R749.5

【共引文献】