一种体外抗β-分泌酶活性快速评价方法的建立与应用

发布时间：2018-06-17 02:19

本文选题：阿尔茨海默氏症 + β-分泌酶　；参考：《中国食品学报》2015年10期

【摘要】：目的:在相对简单的试验条件下建立一种快速筛选具有抑制β-分泌酶活性的生物活性物质的分析方法,为快速筛选具有潜在抗阿尔茨海默氏症(AD)活性的天然产物提供技术基础。方法:以HPLC为分析手段,以β-分泌酶在单位时间内反应底物减少量为检测依据,测定不同天然产物的抑制β-分泌酶活性作用。结果:测定30 min内3种食源性天然产物——壳寡糖、茶氨酸、姜黄素对β-分泌酶在单位时间内消耗反应底物的抑制。壳寡糖的有效质量浓度范围为0.01~0.5 mg/m L,IC50为0.1 mg/m L;茶氨酸的有效剂量达到100 mmol/L;姜黄素有效浓度范围为20~50μmol/L。结论:本方法可用于在我国丰富的食品及中药资源中快速筛选β-分泌酶抑制剂。其操作简便,结果稳定,可重复性好,具备较为广泛的样本适用性,特别是分子质量低于1.5 ku任意溶解度的食源性化合物。
[Abstract]:Objective: to establish a rapid method for screening bioactive substances with 尾 -secretase activity under relatively simple experimental conditions, and to provide a technical basis for rapid screening of natural products with potential anti-AD activity. Methods: the inhibition of 尾 -secretase activity by different natural products was determined by HPLC, based on the reduction of substrates of 尾 -secretase in a unit time. Results: the inhibition of 尾 -secretory enzymes by chitosan, theanine and curcumin in 30 min was determined. The effective mass concentration range of chitosan oligosaccharide is 0.01g / L 0.5 mg/m / L, the effective dose of theanine is 100mmol / L, and the effective concentration range of curcumin is 200.50 渭 mol / L. Conclusion: this method can be used for rapid screening of 尾-secretase inhibitors in Chinese food and traditional Chinese medicine resources. The method is simple, stable, reproducible and suitable for a wide range of samples, especially foodborne compounds with molecular weight less than 1.5 ku.
【作者单位】：北京联合大学生物活性物质与功能食品北京市重点实验室;北京联合大学应用文理学院;
【基金】：国家自然科学基金项目(31471587) 北京市教委科技发展面上项目(KM201311417013)
【分类号】：R749.16

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