VDR基因型与精神分裂症及利培酮诱导代谢变化的相关性

发布时间：2018-06-22 22:26

本文选题：VDR + 基因多态性　；参考：《中南大学》2013年硕士论文

【摘要】：一、目的探讨VDR基因多态性与中国精神分裂症的相关性；比较首发精神分裂症患者服用利培酮前和4周后胰岛素与血脂四项水平的差异,并研究胰岛素和血脂水平变化与VDR基因多态性的相关性。二、方法 1.基因分型采用PCR-LDR技术对VDR基因的四个位点Fok-I(RS2228570)、 Cdx-2(RS11568820)、Bsm-I(RS1544410)、Apa-I(RS7975232)进行分型。 2.VDR基因多态性与中国精神分裂症相关性研究收集精神分裂症患者222例作为病例组,150例健康体检者作为健康对照组,分别对病例组和对照组的VDR基因进行分型和统计比较。 3.VDR基因多态性与利培酮诱导胰岛素和血脂变化的相关性研究选择首次发病、未服用抗精神病药的住院精神分裂症患者29例,单用利培酮治疗,分别检测服药前和服药4周后的空腹胰岛素和血脂四项水平,并对这29例病例VDR基因进行分型。所有数据统计均采用SPSS20.0软件进行。三、结果 1.VDR基因多态性与精神分裂症的相关性病例组和对照组的VDR基因型和基因频率的分布无显著差异；相同性别的病例组和对照组基因分布无显著差异；各基因型的平均发病年龄无显著差异；男性和女性平均发病年龄分别为(21.99±6.05)岁和(24.42±7.97)岁,差异无统计学意义(P=0.121)。 2.VDR基因多态性与利培酮诱导胰岛素和血脂变化的相关性服用利培酮4周后,胰岛素和甘油三酯水平显著升高(p0.05)。Fok-Ⅰ基因的不同基因型,胰岛素水平存在显著性差异(0周P=0.019,4周P=0.002),利培酮治疗后胰岛素变化也具有显著差异(P=0.005),CC基因型患者胰岛素升高最显著；不同Apa-I基因型,服药4周后TG水平具有显著差异(P=0.030),利培酮治疗后TC(P=0.028)和TG变化(P=0.041)存在显著差异,AA基因型携带者TC和TG变化较其他基因型更大。四、结论 1.VDR基因的四个位点Fok-I、Cdx-2、Bsm-I、Apa-I与中国精神分裂症无显著相关性。 2.服用利培酮4周后胰岛素和TG水平显著升高。 3.Fok-I与利培酮诱导的胰岛素升高相关,CC基因型胰岛素升高最大；Apa-I基因的AA基因型携带者更容易产生血脂异常的不良反应。
[Abstract]:First, the purpose
To investigate the correlation between VDR gene polymorphism and Chinese schizophrenia, compared the four levels of insulin and blood lipid levels before and 4 weeks after risperidone in patients with schizophrenia, and the correlation between the changes of insulin and blood lipids and the polymorphism of VDR gene.
Two, method
1. genotyping
PCR-LDR technology was used to classify the four loci of VDR gene Fok-I (RS2228570), Cdx-2 (RS11568820), Bsm-I (RS1544410) and Apa-I (RS7975232).
Association between 2.VDR gene polymorphism and schizophrenia in China
222 cases of schizophrenic patients were collected as case group and 150 healthy subjects were used as healthy control group. The VDR gene of case group and control group were divided and compared.
Association of 3.VDR gene polymorphisms with risperidone induced changes in insulin and blood lipids
29 hospitalized schizophrenic patients who had not taken antipsychotic drugs for the first time were selected and treated with risperidone alone. Four levels of fasting insulin and blood lipid were detected before and 4 weeks after taking medicine, and the VDR gene was typed in these 29 cases.
All data statistics are carried out by SPSS20.0 software.
Three, the result
Association of 1.VDR gene polymorphism with schizophrenia
There was no significant difference in the distribution of VDR genotypes and gene frequencies between the case group and the control group, and there was no significant difference in the distribution of gene distribution in the same sex group and the control group; the average age of onset of the genotypes had no significant difference, and the average age of the male and female was (21.99 + 6.05) and (24.42 + 7.97) years, respectively (P =0.121).
Association of 2.VDR gene polymorphisms with risperidone induced changes in insulin and blood lipids
After 4 weeks of risperidone, the levels of insulin and triglyceride increased significantly (P0.05) in different genotypes of.Fok- I gene, the insulin level was significantly different (0 weeks P=0.019,4 weeks P=0.002), and the changes of insulin after risperidone were also significantly different (P=0.005), and the most significant increase in insulin in the CC genotype patients; the different Apa-I genotypes, After 4 weeks, the level of TG was significantly different (P=0.030). There was a significant difference in TC (P=0.028) and TG changes (P=0.041) after risperidone treatment. The TC and TG changes of the AA genotype carriers were larger than those of the other genotypes.
Four. Conclusion
There are no significant correlations between the four loci of 1.VDR gene Fok-I, Cdx-2, Bsm-I, Apa-I and schizophrenia in China.
2. risperidone increased the level of insulin and TG after 4 weeks of risperidone treatment.
3.Fok-I is associated with risperidone induced insulin rise, and CC genotype insulin increases most; the AA genotype carriers of the Apa-I gene are more likely to produce adverse reactions to dyslipidemia.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R749.3

【参考文献】