吲哚胺2,3-双加氧酶在创伤后应激障碍大鼠海马中的表达变化与作用

发布时间：2018-06-23 13:10

  本文选题：创伤后应激障碍 + 肿瘤坏死因子-α　； 参考：《华中科技大学学报(医学版)》2016年04期

【摘要】：目的通过检测创伤后应激障碍(post-traumatic stress disorder,PTSD)大鼠海马中吲哚胺2,3-双加氧酶(indoleamine 2,3-dioxygenase,IDO)含量变化与运用IDO抑制剂治疗对神经元细胞的保护机制,探讨PTSD发病原因及机制。方法将60只健康雄性Wistar大鼠随机分为:对照组、PTSD组、PTSD+IDO抑制剂治疗组,运用ELISA试剂盒检测肿瘤坏死因子-α(tumor necrosis factor,TNF-α)、白介素-6(interleukin-6,IL-6)含量变化,通过RT-PCR、Western blot检测IDO的表达情况。通过TUNEL染色法检测海马神经元凋亡率,并对大鼠进行行为学评估。结果检测结果显示对照组、PTSD组与IDO抑制剂治疗组TNF-α分别为[(1.26±0.12)vs.(8.58±0.67)vs.(3.69±0.41)pg/mL]、IL-6分别为[(2.28±0.19)vs.(15.72±1.42)vs.(7.45±0.58)pg/mL]、IDOmRNA分别为[(0.152 7±0.014 7)vs.(0.827 8±0.079 6)vs.(0.223 6±0.038 7)]、IDO蛋白分别为[(0.061 2±0.008 6)vs.(1.232 9±0.114 8)vs.(0.423 5±0.041 1)]、神经元的凋亡率为[(5.46±1.87)%vs.(81.47±6.86)%vs.(42.54±3.98)%](均P0.05),行为学表现明显改善。结论 PTSD大鼠海马区域TNF-α、IL-6、IDO表达显著提高,IDO抑制剂治疗能降低其含量,细胞因子、IDO在PTSD发病机制中起重要作用,运用IDO抑制剂能改善PTSD大鼠海马区域神经元损伤。
[Abstract]:Objective to investigate the changes of indoleamine 3-dioxygenase (indoleamine _ 2) _ 3-dioxygenase (IDO) content in hippocampus of rats with post-traumatic stress disorder (post-traumatic stress) and the protective mechanism of IDO inhibitor therapy on neuronal cells, and to explore the pathogenesis and mechanism of the pathogenesis of indoleamine 3-dioxygenase (IDO) in the hippocampus of rats with post-traumatic stress disorder (PTSD). Methods Sixty healthy male Wistar rats were randomly divided into two groups: the control group was treated with PTSD-IDO inhibitor. The levels of tumor necrosis factor- 伪 (tumor necrosis factor-TNF- 伪 and interleukin-6 (IL-6) were detected by Elisa kit, and the expression of Ido was detected by RT-PCR- Western blot. The apoptotic rate of hippocampal neurons was detected by Tunel staining, and the behavior of rats was evaluated. 缁撴灉妫，

本文编号：2057219