慢性应激诱导的抑郁大鼠纹状体DRD1和miR-504的表达

发布时间：2018-06-28 22:35

本文选题：慢性不可预知性应激 + 抑郁样行为　；参考：《中国临床心理学杂志》2014年04期

【摘要】：目的:研究慢性不可预知性应激诱导的抑郁大鼠纹状体内多巴胺受体D1型(dopamine receptor D1,DRD1)和micro RNA-504(mi R-504)的表达,探讨mi R-504是否介导了DRD1基因表达参与调控慢性不可预知性应激诱发大鼠抑郁的发生。方法:将10周龄雄性SD大鼠随机分为实验组和对照组。实验组大鼠接受连续21天慢性不可预知性应激,对照组不接受任何实验性处理。两组大鼠均于13周龄时,采用强迫游泳测验、糖水偏爱测验和旷场试验评定大鼠的抑郁水平,以实时定量荧光RT-PCR(q RT-PCR)检测纹状体内DRD1 m RNA和mi R-504的表达水平,以免疫印迹法(Western Blot)检测纹状体DRD1蛋白质表达水平。结果:与对照组大鼠相比,实验组大鼠直立次数和爬行总路程均减少,而被动漂浮时间延长;两组大鼠的大便颗数和糖水偏爱率差异均不显著。实验组大鼠纹状体内DRD1蛋白和mi R-504表达量均降低,而DRD1 m RNA的表达量与对照组比较差异不显著。皮尔逊相关分析显示,两组大鼠纹状体内DRD1蛋白水平与大鼠的被动漂浮时间呈负相关,而与其他的行为测验水平无明显相关;两组大鼠纹状体内mi R-504水平与行为测验水平均无明显相关。结论:慢性不可预知性应激诱导大鼠产生了抑郁样行为并能降低纹状体DRD1蛋白和mi R-504的表达;mi R-504可能并不参与纹状体内DRD1基因表达参与调控慢性不可预知性应激诱发大鼠抑郁的发生过程。
[Abstract]:Objective: to study the expression of dopamine receptor D1 (dopamine receptor D1DRD1 and micro RNA-504 (mi R-504) in the striatum of depression rats induced by chronic unpredictable stress. To investigate whether mi R-504 mediates DRD1 gene expression in the regulation of depression induced by chronic unpredictable stress in rats. Methods: ten-week old male SD rats were randomly divided into experimental group and control group. Rats in the experimental group received chronic unpredictable stress for 21 days, while the control group did not receive any experimental treatment. At the age of 13 weeks, the depression level was assessed by forced swimming test, sugar water preference test and open field test. The expression of DRD1 mRNA and miR-504 in the striatum were detected by real-time quantitative RT-PCR (Q RT-PCR). The expression of DRD1 protein in striatum was detected by Western blot. Results: compared with the control group, the rats in the experimental group had fewer times of standing up and the total distance of crawling, but the passive floating time was prolonged, and there was no significant difference in the number of feces and the preference rate of sugar and water between the two groups. The expression of DRD1 protein and miR-504 in the striatum of the experimental group was decreased, but the expression of DRD1 mRNA was not significantly different from that of the control group. Pearson correlation analysis showed that there was a negative correlation between DRD1 protein level and passive floating time in the striatum of the two groups, but no significant correlation between DRD1 protein level and other behavioral test levels. There was no significant correlation between the level of MI R-504 in striatum and the level of behavioral test in the two groups. Conclusion: chronic unpredictable stress induced depression like behavior in rats and decreased the expression of DRD1 protein and mi R-504 in striatum. [WT5 "HZ] [WT5" BZ] [WT5 "BZ] [WT5" BZ] the expression of DRD1 gene in the striatum may not be involved in the regulation of chronic unpredictable stress. The process of induced depression in rats.
【作者单位】：中南大学湘雅二医院医学心理研究所;
【基金】：国家自然科学基金项目(81071097) 湖南省研究生科研创新项目(CX2013B113)
【分类号】：R749.4

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