超低频经颅磁刺激对脑缺血大鼠认知功能的影响及机制

发布时间：2018-07-04 13:51

本文选题：经颅磁刺激 + 脑缺血　；参考：《重庆医学》2016年21期

【摘要】：目的研究超低频经颅磁刺激(TMS)对脑缺血大鼠认知功能的影响并探讨其机制。方法将60只健康大鼠分为4组(各15只):A组(假手术组),B组(模型组),C组(TMS组)和D组(TMS+H89组)。比较各组大鼠的逃避潜伏时间、跨越原平台次数及血管内皮生长因子(VEGF)、脑源性神经营养因子(BDNF)和巢蛋白(nestin)的表达水平。结果 A组大鼠逃避潜伏期为(16.31±2.33)s,跨越原平台次数为(8.02±1.76)次;B组大鼠逃避潜伏期为(57.14±2.89)s,跨越原平台次数为(3.15±0.88)次;C组大鼠逃避潜伏期为(29.18±1.95)s,跨越原平台次数为(5.44±0.75)次;D组大鼠逃避潜伏期为(45.87±2.06)s,跨越原平台次数为(4.16±1.02)次。与A组比较,B、C、D组大鼠的逃避潜伏期明显延长,且B组长于D组及C组,差异均有统计学意义(P0.05);大鼠的跨越原平台次数明显减少,且B组少于D组及C组,差异均有统计学意义(P0.05)。各组大鼠VECF、BDNF和nestin表达水平比较,差异均有统计学意义(P0.05)。结论低频TMS可以明显改善脑缺血大鼠的认知功能,其作用与环磷腺苷效应元件结合蛋白及其下游基因(VEGF、BDNF)的表达有关。
[Abstract]:Objective to study the effect of ultra low frequency transcranial magnetic stimulation (TMS) on cognitive function and its mechanism in rats with cerebral ischemia. Methods Sixty healthy rats were divided into 4 groups (15 rats each): group A (sham operation group), group B (model group), group C (group TMS) and group D (TMS H89). The escape latency, the times of crossing the original platform and the expression levels of vascular endothelial growth factor (VEGF), brain-derived neurotrophic factor (BDNF) and nestin (nestin) were compared. Results the escape latency was (16.31 卤2.33) s in group A, (8.02 卤1.76) times in group B, (57.14 卤2.89) s in group B, (3.15 卤0.88) times in group C and (29.18 卤1.95) s in group C, and (5.44 卤0.75) times in group D. The escape latency was (45.87 卤2.06) s, and the times of crossing the original platform was (4.16 卤1.02) times. Compared with group A, the escape latency in group B was significantly longer than that in group D and group C (P0.05), and the frequency of crossing the original platform was significantly decreased in group B than that in group D and group C. the difference was statistically significant (P0.05). The expression levels of BDNF and nestin in VECF were significantly higher than those in control group (P0.05). Conclusion low frequency TMS can significantly improve the cognitive function of rats with cerebral ischemia, which is related to the expression of cyclic adenosine effector element binding protein and its downstream gene (VEGFN BDNF).
【作者单位】：川北医学院附属医院神经内科;川北医学院附属医院心血管内科;
【基金】：国家自然科学基金资助项目(81070101)
【分类号】：R749.13

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