糖调节受损大鼠认知障碍及脑组织NF-κBp65、TNF-α的表达

发布时间：2018-07-15 19:04

【摘要】：目的观察糖调节受损大鼠脑组织NF-κBp65、TNF-α及NF-κB mRNA、TNF-αmRNA表达,探讨糖耐量受损大鼠的炎症机制及其对认知功能障碍的影响。方法将Wistar大鼠随机分为对照组和实验组,高脂、高糖饲养法建立糖耐量受损大鼠模型;采用免疫组织化学和原位杂交的方法分别从蛋白水平和转录水平检测糖调节受损组与糖耐量正常组大鼠脑组织NF-κBp65、NF-κB mRNA和TNF-α、TNF-αmRNA的表达;Morris水迷宫法评定两组大鼠认知功能。结果与NGT组比较,IGR组第10周NF-κBp65、NF-κB mRNA高表达;第15周TNF-α、TNF-αmRNA高表达。两组间学习记忆能力在第5周无差异(P0.05);在第10周IGR组优于NGT组;第15、第20周实验组逐次下降(P0.05),低于同时点NGT组(P0.05)。Pearson相关分析显示NF-κBp65、NF-κB mRNA和TNF-α、TNF-αmRNA高表达与大鼠认知障碍相关。结论糖调节受损与大鼠认知障碍相关,NF-κB调控的炎症反应可能是其重要机制。
[Abstract]:Objective to investigate the expression of NF- 魏 B p65 TNF- 伪 and NF- 魏 B mRNA-tNF- 伪 mRNA in rats with impaired glucose tolerance, and to explore the inflammatory mechanism and its effect on cognitive impairment in rats with impaired glucose tolerance. Methods Wistar rats were randomly divided into two groups: control group and experimental group. The expression of NF- 魏 B p65 and TNF- 伪 TNF- 魏 B mRNA and TNF- 伪 TNF- 伪 mRNA in the brain of impaired glucose regulated group and normal glucose tolerance group were detected by immunohistochemistry and in situ hybridization respectively. The cognitive function of the two groups was evaluated by Morris water maze. Results compared with NGT group, the expression of NF- 魏 B p65 and TNF- 伪 mRNA in IGR group was higher than that in NGT group at 10 weeks and 15 weeks, respectively. There was no difference in learning and memory ability between the two groups at the 5th week (P0.05); IGR group was superior to NGT group at the 10th week; at the 15th and 20th week, the experimental group decreased one after another (P0.05), which was lower than NGT group (P0.05) .Pearson correlation analysis showed that the high expression of NF- 魏 B p65 and TNF- 伪 TNF- 伪 mRNA was associated with cognitive impairment in rats. Conclusion impaired glucose regulation and cognitive impairment may play an important role in the regulation of NF- 魏 B inflammation in rats.
【作者单位】：天津医科大学第四中心临床学院神经内科;
【分类号】：R749.2

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