神经干细胞移植治疗老年痴呆

发布时间：2018-07-27 18:08

【摘要】：背景:神经干细胞移植已应用于临床治疗脑瘫等脑损伤疾病,但对老年痴呆的相关研究并未深入。目的:观察神经干细胞移植对老年痴呆大鼠行为学及免疫调节系统的影响。方法:选取SD大鼠35只,切开脑后皮肤,在脑内海马区注射鹅膏蕈酸,建立老年痴呆模型;另取SD大鼠10只,切开脑后皮肤,在脑内海马区注射生理盐水,作为假手术组。将造模成功的32只老年痴呆大鼠随机分为2组,每组16只,实验组于脑内海马区注射5×109 L-1的同种异体神经干细胞悬液;模型组仅穿刺,不注射任何物质,移植后4周,对3组大鼠进行Morris水迷宫实验,连续5 d;水迷宫实验1周后,检测3组大鼠脑组织匀浆白细胞介素1、白细胞介素10水平,观察脑组织病理形态学变化。结果与结论:1Morris水迷宫实验:模型组认知和记忆能力均明显低于假手术组(P0.01),实验组空间学习能力、记忆能力均显著高于模型组(P0.05,P0.01)。2脑组织匀浆炎炎性因子:模型组白细胞介素1、白细胞介素10水平明显高于假手术组(P0.01),实验组白细胞介素1、白细胞介素10水平明显低于模型组(P0.01)。3脑组织病理:模型组神经元细胞明显少于假手术组,实验组神经细胞数目明显多于模型组。4结果表明,神经干细胞移植对老年性痴呆大鼠神经元具有补充和保护作用,可明显改善动物的学习和记忆能力。
[Abstract]:Background: neural stem cell transplantation has been applied to the treatment of cerebral palsy and other brain injury diseases. Aim: to observe the effect of neural stem cell transplantation on behavior and immune regulatory system in senile dementia rats. Methods: 35 Sprague-Dawley (SD) rats were selected to cut back the skin of the brain, and the model of senile dementia was established by injecting muscaric acid into the hippocampal area of the brain, and 10 SD rats were selected as sham-operated group, 10 rats were cut off the skin of the brain and the normal saline was injected into the hippocampal area of the brain. Thirty-two senile dementia rats were randomly divided into two groups, 16 rats in each group. The experimental group was injected with 5 脳 109 L-1 neural stem cell suspension in the hippocampal area of the brain, and the model group was only punctured without any injection of any substance at 4 weeks after transplantation. The Morris water maze test was carried out for 5 days, and the levels of interleukin 1 and interleukin 10 in the brain homogenate of the three groups were measured after 1 week of water maze test, and the pathomorphological changes of brain tissue were observed. Results and conclusion: the cognitive and memory abilities of the model group were significantly lower than those of the sham-operated group (P0.01), and the spatial learning ability of the experimental group was significantly lower than that of the control group (P0.01). The level of IL-1 and IL-10 in model group were significantly higher than those in sham-operation group (P0.01). The levels of IL-1 and IL-10 in experimental group were significantly lower than those in model group (P0.05, P0.01), and the levels of IL-1 and IL-10 in model group were significantly higher than those in sham-operated group (P0.01). In the model group (P0.01) .3, the number of neurons in the model group was significantly lower than that in the sham-operated group. The number of nerve cells in the experimental group was significantly higher than that in the model group. The results showed that the neural stem cell transplantation could supplement and protect the neurons of senile dementia rats, and could obviously improve the learning and memory ability of the animals.
【作者单位】：邯郸市第一医院老年病二科;河北大学附属医院老年病科;
【基金】：河北省医学科学研究重点课题(20130921)~~
【分类号】：R749.16

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