通络醒脑泡腾片对大鼠慢性脑缺血致学习记忆功能损伤的影响

发布时间：2018-07-31 15:56

【摘要】：目的:考察通络醒脑泡腾片对慢性脑缺血致学习记忆障碍模型大鼠学习记忆及海马Na+-K+-ATP酶表达的影响。方法:采用双侧颈总动脉结扎法(2-VO)制作SD种大鼠慢性脑缺血致学习记忆障碍模型。将造模成功大鼠50只随机分为模型对照组、甲磺酸双氢麦角碱片组(0.7 mg·kg-1)、通络醒脑泡腾片高、中、低剂量组(7.56,3.78,1.59 g·kg-1),另取10只假手术动物作为对照组。10 mL·kg-1·d-1连续灌胃90 d。给药第86天进行Morris水迷宫训练,第90天进行学习记忆能力测试;脑组织用10%甲醛固定,常规制片、染色后进行病理形态学观察;采用免疫组化和图像分析技术检测海马区Na+-K+-ATP酶的表达。结果:通络醒脑泡腾片各给药组与模型组比较均能显著缩短训练第5天的逃避潜伏期,显著增加第一象限进入次数、经过逃逸平台次数和进入有效区域次数(P0.05);病理形态学检测表明,模型组病理积分极显著高于假手术组(P0.01),中剂量组较模型组病理积分显著降低(P0.05)。免疫组织化学检测显示模型组Na+-K+-ATP酶平均吸光度较假手术组显著降低(P0.05),高、中剂量组的平均吸光度较模型组极显著增高(P0.01)。结论:通络醒脑泡腾片具有改善慢性脑缺血所致学习记忆障碍模型大鼠学习记忆能力、减轻海马组织病变,促进海马区Na+-K+-ATP酶表达的作用。
[Abstract]:Objective: To investigate the effect of Tongluo Xingnao effervescent tablet on learning memory and hippocampal Na+-K+-ATP enzyme expression in rats with learning and memory impairment induced by chronic cerebral ischemia. Methods: using bilateral common carotid artery ligation (2-VO) to make model of learning and memory impairment induced by chronic cerebral ischemia in SD rats. 50 rats were randomly divided into model control group, a model control group. The group (0.7 mg. Kg-1), Tongluo Xingnao effervescent tablet was high, middle and low dose group (7.56,3.78,1.59 G. Kg-1). Another 10 sham animals were taken as control group.10 mL. Kg-1 D-1 continuous 90 D. for eighty-sixth days for Morris water maze training. The learning and memory ability test was performed on ninetieth days; the brain tissue was fixed with 10% formaldehyde, routine routine. The expression of Na+-K+-ATP enzyme in the hippocampus was detected by immunohistochemistry and image analysis. Results: all the drug groups of Tongluo Xingnao effervescent tablet and model group could significantly shorten the escape incubation period of fifth days, significantly increase the number of first quadrant entry, the number of escape platforms and entry. The number of effective area (P0.05), pathological examination showed that the pathological score of the model group was significantly higher than that of the sham group (P0.01), and the medium dose group was significantly lower than the model group (P0.05). The average absorbency of the Na+-K+-ATP enzyme in the model group was significantly lower than that of the sham group (P0.05), high, and the average absorption of the medium dose group. It is significantly higher than the model group (P0.01). Conclusion: Tongluo Xingnao effervescent tablet can improve the learning and memory ability of learning and memory impairment model rats induced by chronic cerebral ischemia, reduce the pathological changes of hippocampus and promote the role of Na+-K+-ATP enzyme expression in the hippocampus.
【作者单位】：四川省中药资源系统研究与开发利用国家重点实验室培育基地;成都中医药大学;四川省医学科学院四川省人民医院;
【基金】：国家“重大新药创制”科技重大专项(2013ZX09103002-008) 四川省杰出青年学术技术带头人资助计划(2011JQ0014) 四川省学术和技术带头人培养资金项目(重点) 四川省“十一五”支撑项目(2010SZ0195) 四川省教育厅自然科学重点项目(12ZA041) 成都市产业发展技术支撑计划——高校院所应用基础研究(12DXYB321JH-002)
【分类号】：R749.16

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