蝙蝠葛苏林碱对阿尔茨海默病小鼠海马的影响和机制

发布时间：2018-08-12 18:31

【摘要】：目的探讨蝙蝠葛苏林碱(DS)对阿尔茨海默病(AD)小鼠影响及机制。方法 C57BL/6小鼠随机分对照组、模型组、多奈哌齐组(0.001 g·kg-1)及DS高、中和低剂量组(1.08、0.54、0.27 g/kg)。采用β-淀粉样蛋白(Aβ)1~42(80 pmol/μl)脑室注射诱导AD模型。治疗30 d后测小鼠学习记忆能力,透射电镜观察海马CA3区,测脏器指数和脑伊文氏蓝(EB),酶联免疫吸附法测海马β-淀粉样蛋白前体蛋白(APP)、Aβ1~42、白介素(IL)-1β、肿瘤坏死因子(TNF)-α、总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及丙二醛(MDA)。结果与模型组比较,DS改善AD小鼠学习记忆能力(P0.05)和海马结构,明显升高脾和胸腺指数(P0.05),其他脏器指数改变不明显(P0.05),EB、APP、Aβ1-42、IL-1β、TNF-α和MDA明显降低(P0.05),T-AOC、SOD和GSH-Px明显升高(P0.05)。结论 DS改善AD小鼠学习记忆及海马结构,增强免疫和抗氧化,抑制APP、Aβ1-42、IL-1β和TNF-α可能是抗AD机制。
[Abstract]:Objective to investigate the effect and mechanism of daurine (DS) on (AD) mice with Alzheimer's disease. Methods C57BL/6 mice were randomly divided into three groups: control group, model group, Donepezil group (0.001 g kg-1) and high, medium and low dose groups (1.08C 0.54U 0.27 g/kg). The AD model was induced by intracerebroventricular injection of 尾 -amyloid protein (A 尾) 1N 42 (80 pmol/ 渭 l). After treatment for 30 days, the ability of learning and memory was measured, and the hippocampal CA3 area was observed by transmission electron microscope. Measurement of organ index and Yi Wen blue (EB), enzyme-linked immunosorbent assay for the detection of hippocampal 尾 -amyloid precursor protein (APP) A 尾 _ 1O _ 2, interleukin (IL) -1 尾, tumor necrosis factor (TNF)-伪, total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA). (MDA) Results compared with the model group, the learning and memory ability (P0.05) and hippocampal structure of AD mice were improved, and the indexes of spleen and thymus were increased (P0.05), while the other organ indexes were not changed significantly (P0.05). The levels of TNF- 伪 and MDA were significantly decreased (P0.05). Conclusion DS can improve the learning and memory and hippocampal structure of AD mice, enhance immunity and antioxidation, and inhibit APP A 尾 1-42, IL-1 尾 and TNF- 伪, which may be the mechanism of anti-AD.
【作者单位】：齐齐哈尔医学院;
【基金】：国家自然科学基金(81173576,81373777,81373599) 教育部重点项目(210068)
【分类号】：R749.16

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