产前应激孕鼠子代抑郁样行为观察及机制探讨

发布时间：2018-09-12 15:51

【摘要】：目的观察产前应激孕鼠子代鼠的抑郁样行为,并探讨产前应激导致子代鼠出现抑郁样行为的作用机制。方法将孕鼠20只随机分观察组和对照组各10只。妊娠第14天(孕鼠进入孕晚期)起对观察组给予束缚应激,45 min/次,3次/d,共束缚7 d。对照组孕鼠孕期不做任何处理,常规饲养。两组子代鼠出生30 d时各取20只(雌雄对半),进行糖水偏爱试验,计算两组雌、雄性子代鼠1%糖水消耗比(1%糖水消耗比表示抑郁性行为严重程度);随之将试验后子代鼠断头处死,采用液相色谱串联质谱分析法检测两组子代鼠海马组织谷氨酸(Glu)、γ-氨基丁酸(GABA)和5-羟色胺(5-HT)。结果观察组雌、雄性子代鼠1%糖水消耗比分别为63.13±3.47和59.67±3.33,对照组分别为79.22±7.07和78.67±4.72;观察组雌、雄性子代鼠1%糖水消耗比与对照组相比,P均0.05。观察组雌、雄子代鼠海马组织Glu的相对含量分别为(24.30±1.00)、(31.22±1.57)ng/g,对照组分别为(21.20±0.90)、(24.01±0.28)ng/g,观察组雌、雄性子代鼠海马组织Glu的相对含量与对照组相比,P均0.05;观察组雌、雄子代鼠海马组织GABA的相对含量分别为(7.42±0.36)、(8.35±0.39)ng/g,对照组分别为(13.28±0.38)、(9.94±0.30)ng/g,观察组雌、雄性子代鼠海马组织GABA的相对含量与对照组相比,P均0.05;观察组雌、雄子代鼠海马组织5-HT的相对含量分别为(0.27±0.02)、(0.26±0.01)ng/g,对照组分别为(0.35±0.03)、(0.29±0.02)ng/g,观察组雌性子代大鼠海马组织5-HT的相对含量与对照组相比,P0.05。结论产前应激可导致子代鼠出现抑郁样行为,其机制可能与产前应激升高子代鼠海马组织Glu含量及降低GABA、5-HT的水平有关。
[Abstract]:Objective to observe the depression-like behavior of offspring of pregnant rats under prenatal stress and to explore the mechanism of depressive behavior induced by prenatal stress. Methods Twenty pregnant rats were randomly divided into observation group (n = 10) and control group (n = 10). From the 14th day of pregnancy (the pregnant rats entered the third trimester of pregnancy), the observation group was given restraint stress for 45 min/ 3 / d for a total of 7 days. The pregnant rats in the control group did not do any treatment during pregnancy. At 30 days after birth, 20 offspring of each group (female and male half) were selected for sugar water preference test, and the two groups of females were calculated. The male offspring had a ratio of 1% sugar to water consumption (1% sugar water consumption ratio indicated the severity of depressive behavior), and then the offspring were killed after the experiment. (Glu), 纬 -aminobutyric acid (GABA) and serotonin (5-HT) in hippocampus of two offspring rats were determined by liquid chromatography tandem mass spectrometry (LC-MS). Results the ratio of 1% sugar to water consumption of female and male offspring was 63.13 卤3.47 and 59.67 卤3.33 in the observation group and 79.22 卤7.07 and 78.67 卤4.72 in the control group, respectively. The relative content of Glu in hippocampal tissue of female and male offspring of observation group was (24.30 卤1.00), (31.22 卤1.57) ng/g, control group was (21.20 卤0.90), () 24.01 卤0.28 ng/g, group, the relative content of Glu in hippocampal tissue of male offspring group was 0.05 compared with that of control group (P < 0.05). The relative content of GABA in hippocampal tissue of male offspring rats was (7.42 卤0.36), (8.35 卤0.39) ng/g, control group was (13.28 卤0.38), (9.94 卤0.30) ng/g, observation group, and the relative content of GABA in hippocampal tissue of male offspring rats was 0.05 compared with that of control group (P < 0.05). The relative content of 5-HT in hippocampus of male offspring was (0.27 卤0.02), (0.26 卤0.01) ng/g, control group was (0.35 卤0.03), () 0.29 卤0.02 ng/g, observation group, the relative content of 5-HT in hippocampus of female offspring group was higher than that of control group (P 0.05). Conclusion Prenatal stress may lead to depressive behavior in offspring, and the mechanism may be related to the increase of Glu content in hippocampus and the decrease of GABA,5-HT in hippocampus.
【作者单位】：西安交通大学医学部;西安交通大学医学部第一附属医院;
【基金】：国家自然科学基金青年基金项目(31200843) 中央高校基本科研业务费专项资金项目(xjj2015077)
【分类号】：R749.4

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