p11mRNA与创伤后应激障碍的相关性研究
发布时间：2018-09-14 09:14
【摘要】：目的:探讨创伤后应激障碍(Post Traumatic Stress Disorder,PTSD)患者外周血单个核细胞p11m RNA水平的变化及其与PTSD患者临床症状的相关性。方法:1.研究对象:收集的病例为2013年10月至2014年12月就诊于新疆某大型三级甲等综合医院的人群,PTSD组为经历创伤事件者,共计34例。非PTSD组为同期经历创伤事件,但未发生PTSD的人群,共计30例。2.研究工具:一般资料调查问卷、创伤后应激障碍量表平民版(Post Traumatic Stress Disorder Checklist Civilian Version,PCL-C)、汉密顿焦虑量表(Hamilton Anxiety Scale,HAMA)、汉密顿抑郁量表(Hamilton Depression Scale,HAMD)。3.研究过程:收集就诊于我科门诊及住院部中经历过严重精神创伤事件的人群,进行一般情况调查问卷、PCL-C、HAMA、HAMD量表资料的收集。对于PTSD的诊断由2名精神科医生完成。将符合入组标准的人群,在入组当日或次日清晨,采集空腹静脉血,待血样本收集完成后送至中心实验室进行试验。4.外周血单个核细胞p11m RNA水平的测定:采用实时荧光定量聚合酶链式反应(Real-time Quantitative Reverse Transcription Polymerase Chain Reaction,QRT-PCR)检测p11m RNA在PTSD组及非PTSD组外周血单个核细胞中表达水平的差异。5.统计方法:使用“上海惠诚心理测验软件”对原始资料进行转化,使用SPSS17.0统计软件进行分析,计数资料的比较采用χ2检验。计量资料进行比较时,若为正态资料,采用t检验;若为非正态资料,采用非参数检验(Nonparametric Tests)Mann-Whitney U检验法。p11m RNA在各组的表达水平与临床特征的相关性采用Spearson相关分析。P0.05双侧检验,为检验有统计学意义。结果:1.p11m RNA在PTSD组的表达水平低于非PTSD组,差异具有统计学意义(P0.05)。2.p11m RNA与PCL-C、HAMA、HAMD量表评分呈负相关(P0.05)。3.p11mRNA与患者年龄、经历创伤事件的年龄及距创伤事件的时间无相关性(P0.05)。结论:1.PTSD患者外周血单个核细胞p11m RNA表达量低于未发生PTSD者,外周血单个核细胞p11m RNA可能是一个区分PTSD患者与非PTSD者的生物学指标。2.经历创伤事件的人群的临床症状越严重,外周血单个核细胞p11m RNA表达量越低。
[Abstract]:Objective: to investigate the changes of p11m RNA in peripheral blood mononuclear cells (PBMC) in patients with post-traumatic stress disorder (Post Traumatic Stress Disorder,PTSD) and its correlation with clinical symptoms of PTSD patients. Method 1: 1. Participants: a total of 34 cases of PTSD were collected from October 2013 to December 2014 in a large Grade 3A General Hospital in Xinjiang. The non PTSD group was a group of 30 patients (30 cases) who had experienced traumatic events but did not have PTSD at the same time. Research tools: general data questionnaire, Post-traumatic stress Disorder (Post Traumatic Stress Disorder Checklist Civilian Version,PCL-C), Hamilton anxiety scale (Hamilton Anxiety Scale,HAMA), Hamilton Depression scale (Hamilton Depression Scale,HAMD). The research process: collect the people who have experienced severe trauma events in outpatient department and inpatient department of our department, and collect the data of PCL-CU Hamah Hamd scale. The diagnosis of PTSD was performed by two psychiatrists. Fasting venous blood was collected on the same day or in the early morning of the next day, and the blood samples were collected and sent to the central laboratory for test. 4. Detection of p11m RNA in peripheral blood mononuclear cells: the expression of p11m RNA in peripheral blood mononuclear cells from PTSD group and non-PTSD group was detected by real-time fluorescence quantitative polymerase chain reaction (Real-time Quantitative Reverse Transcription Polymerase Chain Reaction,QRT-PCR). Statistical methods: the original data were transformed by "Shanghai Huicheng Psychological Test Software" and analyzed by SPSS17.0 statistical software. 蠂 ~ 2 test was used to compare the counting data. When the measurement data were compared, t test was used for normal data, and for non-normal data, the expression level of p11m RNA in each group was tested by (Nonparametric Tests) Mann-Whitney U test. The correlation between the expression level of p11m RNA in each group and clinical characteristics was analyzed by Spearson correlation analysis. P05 bilateral test was used. There is statistical significance for the test. Results the expression of p11m RNA in PTSD group was significantly lower than that in non-PTSD group (P0.05). There was a negative correlation between p11m RNA and PCL-C,HAMA,HAMD score (P0.05) .3.p11mmRNA had no correlation with patient's age, the age of traumatic event and the time to traumatic event (P0.05). Conclusion: 1. The expression of p11m RNA in peripheral blood mononuclear cells in patients with PTSD is lower than that in patients without PTSD. The expression of p11m RNA in peripheral blood mononuclear cells may be a biological marker to distinguish PTSD patients from non-PTSD patients. The more severe the clinical symptoms, the lower the expression of p11m RNA in peripheral blood mononuclear cells.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R749.5
本文编号：2242257
[Abstract]:Objective: to investigate the changes of p11m RNA in peripheral blood mononuclear cells (PBMC) in patients with post-traumatic stress disorder (Post Traumatic Stress Disorder,PTSD) and its correlation with clinical symptoms of PTSD patients. Method 1: 1. Participants: a total of 34 cases of PTSD were collected from October 2013 to December 2014 in a large Grade 3A General Hospital in Xinjiang. The non PTSD group was a group of 30 patients (30 cases) who had experienced traumatic events but did not have PTSD at the same time. Research tools: general data questionnaire, Post-traumatic stress Disorder (Post Traumatic Stress Disorder Checklist Civilian Version,PCL-C), Hamilton anxiety scale (Hamilton Anxiety Scale,HAMA), Hamilton Depression scale (Hamilton Depression Scale,HAMD). The research process: collect the people who have experienced severe trauma events in outpatient department and inpatient department of our department, and collect the data of PCL-CU Hamah Hamd scale. The diagnosis of PTSD was performed by two psychiatrists. Fasting venous blood was collected on the same day or in the early morning of the next day, and the blood samples were collected and sent to the central laboratory for test. 4. Detection of p11m RNA in peripheral blood mononuclear cells: the expression of p11m RNA in peripheral blood mononuclear cells from PTSD group and non-PTSD group was detected by real-time fluorescence quantitative polymerase chain reaction (Real-time Quantitative Reverse Transcription Polymerase Chain Reaction,QRT-PCR). Statistical methods: the original data were transformed by "Shanghai Huicheng Psychological Test Software" and analyzed by SPSS17.0 statistical software. 蠂 ~ 2 test was used to compare the counting data. When the measurement data were compared, t test was used for normal data, and for non-normal data, the expression level of p11m RNA in each group was tested by (Nonparametric Tests) Mann-Whitney U test. The correlation between the expression level of p11m RNA in each group and clinical characteristics was analyzed by Spearson correlation analysis. P05 bilateral test was used. There is statistical significance for the test. Results the expression of p11m RNA in PTSD group was significantly lower than that in non-PTSD group (P0.05). There was a negative correlation between p11m RNA and PCL-C,HAMA,HAMD score (P0.05) .3.p11mmRNA had no correlation with patient's age, the age of traumatic event and the time to traumatic event (P0.05). Conclusion: 1. The expression of p11m RNA in peripheral blood mononuclear cells in patients with PTSD is lower than that in patients without PTSD. The expression of p11m RNA in peripheral blood mononuclear cells may be a biological marker to distinguish PTSD patients from non-PTSD patients. The more severe the clinical symptoms, the lower the expression of p11m RNA in peripheral blood mononuclear cells.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R749.5
【参考文献】
相关期刊论文 前2条
1 申远;吴文源;李春波;李清伟;;澳大利亚成人急性应激障碍和创伤后应激障碍治疗指南[J];国际精神病学杂志;2008年03期
2 陈耿谊;高镇松;陈政雄;;创伤后应激障碍患者的临床特点及社会支持分析[J];河北医学;2010年01期
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