美满霉素对阿尔茨海默病大鼠认知能力和海马突触可塑性的影响
发布时间：2018-09-17 12:35
【摘要】：目的观察美满霉素对阿尔茨海默病(Alzheimer's disease,AD)大鼠空间学习记忆和海马长时程增强(long-term potentiation,LTP)以及N-甲基-D-天冬氨酸受体2B亚基(N-methyl-D-aspartate receptor subunit 2B,NR2B)表达的影响,探讨美满霉素对AD大鼠神经保护的作用机制。方法选用健康雄性SD大鼠36只,随机分为对照组、模型组、美满霉素治疗组,每组12只。通过侧脑室注射Aβ25-35制作AD大鼠模型。Morris水迷宫检测各组大鼠空间学习记忆能力;在体电生理法记录各组大鼠海马LTP的诱导情况;免疫荧光组织化学法检测各组大鼠海马NR2B的表达。结果与对照组相比,模型组平均逃避潜伏期和第一次到达原平台的时间明显延长,穿环次数明显减少(P0.05),海马LTP幅度明显降低,NR2B表达明显减少(P0.05);与模型组相比,美满霉素治疗组平均逃避潜伏期和第一次到达原平台的时间明显缩短,穿环次数明显增加(P0.05),海马LTP幅度明显升高,NR2B表达明显增加(P0.05)。结论美满霉素可以通过促进海马LTP的形成,上调NR2B的表达,改善AD大鼠认知功能障碍。
[Abstract]:Objective to observe the effects of minocycline on spatial learning and memory, hippocampal long-term potentiation (long-term potentiation,LTP) and the expression of N- methyl-Daspartic acid receptor 2B subunit (N-methyl-D-aspartate receptor subunit _ 2Bn NR2B) in rats with Alzheimer's disease (Alzheimer's disease,AD). To investigate the neuroprotective mechanism of minocycline on AD rats. Methods Thirty-six healthy male SD rats were randomly divided into control group, model group and minocycline treatment group with 12 rats in each group. The AD rat model was made by intracerebroventricular injection of A 尾 25-35. The spatial learning and memory ability of each group was measured by Morris water maze, the induction of hippocampal LTP in each group was recorded by electrophysiological method, and the expression of NR2B in hippocampus was detected by immunofluorescence histochemistry. Results compared with the control group, the average escape latency and the first time to reach the original platform in the model group were significantly prolonged, the number of annular piercing was significantly decreased (P0.05), the amplitude of LTP in hippocampus was significantly decreased (P0.05), and the expression of NR2B was significantly decreased in the model group (P0.05). In minocycline treatment group, the average escape latency and the first time to reach the original platform were significantly shortened, the number of annular piercing increased significantly (P0.05), and the amplitude of LTP in hippocampus increased significantly (P0.05), and the expression of NR2B in hippocampus increased significantly (P0.05). Conclusion minocycline can improve the cognitive function of AD rats by promoting the formation of LTP and up-regulating the expression of NR2B.
【作者单位】： 漯河医学高等专科学校;
【基金】：2015年度河南省科技厅基础与前沿技术研究(152300410014) 2014年度河南省科技厅自然科学资助项目(142300410431) 漯河医学高等专科学校2015年度基础科学重点研究项目(2015-S-LMC06)
【分类号】：R749.16
本文编号：2245942
[Abstract]:Objective to observe the effects of minocycline on spatial learning and memory, hippocampal long-term potentiation (long-term potentiation,LTP) and the expression of N- methyl-Daspartic acid receptor 2B subunit (N-methyl-D-aspartate receptor subunit _ 2Bn NR2B) in rats with Alzheimer's disease (Alzheimer's disease,AD). To investigate the neuroprotective mechanism of minocycline on AD rats. Methods Thirty-six healthy male SD rats were randomly divided into control group, model group and minocycline treatment group with 12 rats in each group. The AD rat model was made by intracerebroventricular injection of A 尾 25-35. The spatial learning and memory ability of each group was measured by Morris water maze, the induction of hippocampal LTP in each group was recorded by electrophysiological method, and the expression of NR2B in hippocampus was detected by immunofluorescence histochemistry. Results compared with the control group, the average escape latency and the first time to reach the original platform in the model group were significantly prolonged, the number of annular piercing was significantly decreased (P0.05), the amplitude of LTP in hippocampus was significantly decreased (P0.05), and the expression of NR2B was significantly decreased in the model group (P0.05). In minocycline treatment group, the average escape latency and the first time to reach the original platform were significantly shortened, the number of annular piercing increased significantly (P0.05), and the amplitude of LTP in hippocampus increased significantly (P0.05), and the expression of NR2B in hippocampus increased significantly (P0.05). Conclusion minocycline can improve the cognitive function of AD rats by promoting the formation of LTP and up-regulating the expression of NR2B.
【作者单位】： 漯河医学高等专科学校;
【基金】：2015年度河南省科技厅基础与前沿技术研究(152300410014) 2014年度河南省科技厅自然科学资助项目(142300410431) 漯河医学高等专科学校2015年度基础科学重点研究项目(2015-S-LMC06)
【分类号】：R749.16
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