阿尔茨海默病患者外周血白细胞介素23和白细胞介素17测定及意义

发布时间：2018-09-18 06:25

【摘要】：目的探讨阿尔茨海默病(Alzheimer’s disease,AD)患者外周血中炎症因子白细胞介素23(Interleukin-23,IL-23)和IL-17水平及其与认知功能损害的相关性。方法纳入AD患者102例(研究组),以及健康老年志愿者100例(对照组)。搜集受试者的一般资料和疾病信息。采用简易精神状态量表(MMSE)和临床痴呆评定量表(CDR)对受试者认知功能和日常行为能力进行评估。应用实时荧光定量逆转录-聚合酶链式反应(RTPCR)检测各组入选者外周血淋巴细胞中IL-23和IL-17的mRNA表达,应用酶联免疫吸附试验(ELISA)检测入选者血清IL-23和IL-17蛋白的表达。比较两组入选者血清炎症因子mRNA及蛋白水平的差异,分析外周血IL-23和IL-17表达的相关性。以全部入选者为研究对象,分析两组IL-17和IL-23表达差异与MMSE评分的相关性。以AD组为研究对象,分析患者IL-17和IL-23表达水平与CDR评分的相关性。结果 AD组外周血淋巴细胞中IL-23和IL-17的mRNA表达水平及血清IL-23和IL-17蛋白的表达水平均显著高于对照组,差异均有统计学意义(P0.05),且两者具有显著正相关性(P0.01)。整体人群中,IL-23和IL-17水平均与MMSE评分呈负相关(P0.05)。AD患者血IL-23和IL-17水平与CDR评分均无相关性。结论外周血IL-23和IL-17水平与整体人群的认知功能相关,但与AD患者的痴呆严重度无明显相关性。
[Abstract]:Objective to investigate the levels of interleukin-23 (Interleukin-23,IL-23) and interleukin-23 (IL-17) in peripheral blood of patients with Alzheimer's disease (Alzheimer's disease,AD) and their correlation with cognitive impairment. Methods 102 AD patients (study group) and 100 healthy elderly volunteers (control group) were enrolled. Collect general information and disease information about the subjects. Simple mental state scale (MMSE) and clinical dementia scale (CDR) were used to evaluate the cognitive function and daily behavior ability of the subjects. The expression of IL-23 and IL-17 in peripheral blood lymphocytes was detected by real-time fluorescence quantitative reverse transcription-polymerase chain reaction (RTPCR), and the expression of IL-23 and IL-17 in serum was detected by enzyme-linked immunosorbent assay (ELISA). The levels of serum inflammatory factor mRNA and protein were compared between the two groups, and the correlation between the expression of IL-23 and IL-17 in peripheral blood was analyzed. The correlation between the expression of IL-17 and IL-23 and the MMSE score was analyzed. The correlation between the expression of IL-17 and IL-23 and CDR score was analyzed in AD group. Results the level of mRNA expression of IL-23 and IL-17 and the expression of serum IL-23 and IL-17 protein in AD group were significantly higher than those in control group (P0.05), and there was a significant positive correlation between them (P0.01). In the whole population, the levels of IL-23 and IL-17 were negatively correlated with the MMSE score (P0.05). There was no correlation between the serum IL-23 and IL-17 levels and the CDR score in AD patients. Conclusion the levels of IL-23 and IL-17 in peripheral blood were correlated with the cognitive function of the whole population, but not with the severity of dementia in the patients with AD.
【作者单位】：遵义医学院第三附属医院老年病科;
【分类号】：R749.16

【相似文献】