DAT1和DRD4基因启动子区甲基化与注意缺陷多动障碍临床症状的关联研究

发布时间：2018-10-08 15:16

【摘要】：目的探索注意缺陷多动障碍(attention deficit hyperactivity disorder,ADHD)患儿多巴胺转运体基因(dopamine transporter gene,DAT1)、多巴胺D4受体基因(dopamine receptor D4 gene,DRD4)启动子区CpG岛甲基化状态与其临床症状严重程度的相关性。方法收集111例ADHD患儿精神类疾病家族史资料,并采用ADHD症状分级父母评定量表(attention deficit hyperactivity disorder rating scale-Ⅳhome version,ADHD-RS-Ⅳ)及自编对立违抗障碍(oppositional defiant disorder,ODD)量表对患者临床症状进行评定。采用亚硫酸氢钠测序法,检测患者外周血的DAT1、DRD4启动子区CpG岛目标片段甲基化状态。结果无抑郁、焦虑和ADHD家族史的患儿DAT1启动子区甲基化水平高于有抑郁、焦虑或ADHD家族史的患儿(P0.05)。DAT1和DRD4甲基化水平在ADHD-RS-Ⅳ总分(≤30分组与30分组)、注意缺陷因子分(≤17分组与17分组)、多动冲动因子分(≤13分组与13分组)低分和高分组的比较中差异均无统计学意义(均P0.05)。ODD量表9分的患儿DAT1甲基化水平较ODD量表得分≥9分的患儿高(P0.05),且患儿DAT1甲基化水平与ODD量表得分呈负相关(r=-2.203,P=0.033)。结论 DAT1启动子区甲基化水平可能影响ADHD患者对立违抗行为的严重程度,且与患者有无抑郁、焦虑和ADHD家族史存在一定关系。
[Abstract]:Objective to explore the correlation between CpG island methylation in dopamine transporter gene (dopamine transporter gene,DAT1) and dopamine D4 receptor gene (dopamine receptor D4 gene,DRD4 promoter in children with attention deficit hyperactivity disorder (attention deficit hyperactivity disorder,ADHD) and the severity of clinical symptoms. Methods the data of family history of mental disorders in 111 cases of ADHD were collected, and the clinical symptoms were evaluated by (attention deficit hyperactivity disorder rating scale- 鈪，

本文编号：2257248