miR-101、APP和COX在SAMP8鼠海马组织中的表达
发布时间：2018-10-18 07:49
【摘要】：目的：miR-101具备APP及COX-2表达双重调节作用的潜能，为探讨miR-101有望成为防治阿尔茨海默病(AD)的新的药物靶点，我们选用快速老化小鼠（SAMP8）做为研究对象，，检测miR-101、APP和COX在不同月龄的SAMP8鼠海马组织中的表达变化状况。 方法：分别取4、8、12月龄SAMP8和对抗老化小鼠SAMR1为研究对象，（1）采用Morris水迷宫实验测试SAMP8鼠的空间记忆能力。（2）半定量RT-PCR检测SAMP8鼠海马组织中APP和COX的mRNA表达变化情况。（3）Western Blot检测SAMP8鼠海马组织中APP和COX的蛋白表达变化情况。（4）real time PCR检测SAMP8鼠海马组织中miR-101的表达变化情况。 结果：（1）Morris水迷宫实验表明8月龄SAMP8鼠即出现空间记忆障碍，12月龄更为显著（p0.01），而SAMR1鼠无显著改变（p0.05）。Aβ42的Western Blot结果与之一致。（2）半定量RT-PCR结果显示SAMP8、SAMR1鼠的海马组织中APP mRNA仅呈轻微增龄性升高，SAMP8组较SAMR1组稍高，但仅在12月龄组出现显著差异。（3）Western Blot结果显示APP在8月龄SAMP8鼠中升高最显著（p0.01），12月龄有下降趋势，而对照组SAMR1鼠却无增龄性显著改变。（4）real time PCR结果显示miR-101在SAMP8鼠中呈显著增龄性降低。（5）各组小鼠COX-2的mRNA和蛋白水平的变化趋势与APP相似，而COX-1的mRNA和蛋白水平均无显著差异。 结论：（1）SAMP8鼠早期即可自发出现空间记忆障碍，并伴有Aβ沉积。（2）SAMP8鼠海马组织中APP和COX-2的表达主要受转录后水平调控。（3）miR-101可能调控APP和COX-2的蛋白表达，而不影响COX-1的表达。（4）miR-101可能成为防治AD的新的药物靶点。
[Abstract]:Objective: miR-101 has the potential to regulate the expression of APP and COX-2. In order to explore the potential of miR-101 as a new drug target for the prevention and treatment of (AD) in Alzheimer's disease, we selected the rapidly aging mouse (SAMP8) as the research object. The expression of miR-101,APP and COX in hippocampal tissue of SAMP8 rats of different months of age was detected. Methods: 12-month-old SAMP8 and anti-aging mice SAMR1 were used as the research objects. (1) Morris water maze test was used to test the spatial memory ability of SAMP8 mice. (2) Semi-quantitative RT-PCR was used to detect the changes of mRNA expression of APP and COX in the hippocampus of SAMP8 rats. (3) the expression of APP and COX in the hippocampus of SAMP8 rats was detected by semi-quantitative RT-PCR. The changes of protein expression of APP and COX in hippocampus of SAMP8 rats were detected. (4) the expression of miR-101 in hippocampus of SAMP8 rats was detected by) real time PCR. Results: (1) Morris water maze test showed that 8 months old SAMP8 rats had spatial memory impairment, especially at 12 months old (p0.01), while SAMR1 mice had no significant change (p0.05). A 尾 42 Western Blot results were consistent with the results of Western Blot). (2) Semi-quantitative RT-PCR results showed APP mRNA in the hippocampus of SAMP8,SAMR1 rats. There was only a slight increase in age, and the SAMP8 group was slightly higher than the SAMR1 group. But there was significant difference only in the 12 month old group. (3) Western Blot results showed that APP increased most significantly in 8 months old SAMP8 mice (p0. 01), and decreased at 12 months old. The results of 4) real time PCR showed that miR-101 decreased significantly in SAMP8 mice. (5) the change trend of mRNA and protein levels of COX-2 in each group was similar to that of APP, but there was no significant difference in mRNA and protein levels in COX-1. Conclusion: (1) the spatial memory disorder and A 尾 deposition can occur spontaneously in SAMP8 rats at early stage. (2) the expression of APP and COX-2 in hippocampus of SAMP8 rats is mainly regulated by posttranscriptional level. (3) miR-101 may regulate the expression of APP and COX-2 protein. (4) miR-101 may be a new drug target for the prevention and treatment of AD.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R749.16
[Abstract]:Objective: miR-101 has the potential to regulate the expression of APP and COX-2. In order to explore the potential of miR-101 as a new drug target for the prevention and treatment of (AD) in Alzheimer's disease, we selected the rapidly aging mouse (SAMP8) as the research object. The expression of miR-101,APP and COX in hippocampal tissue of SAMP8 rats of different months of age was detected. Methods: 12-month-old SAMP8 and anti-aging mice SAMR1 were used as the research objects. (1) Morris water maze test was used to test the spatial memory ability of SAMP8 mice. (2) Semi-quantitative RT-PCR was used to detect the changes of mRNA expression of APP and COX in the hippocampus of SAMP8 rats. (3) the expression of APP and COX in the hippocampus of SAMP8 rats was detected by semi-quantitative RT-PCR. The changes of protein expression of APP and COX in hippocampus of SAMP8 rats were detected. (4) the expression of miR-101 in hippocampus of SAMP8 rats was detected by) real time PCR. Results: (1) Morris water maze test showed that 8 months old SAMP8 rats had spatial memory impairment, especially at 12 months old (p0.01), while SAMR1 mice had no significant change (p0.05). A 尾 42 Western Blot results were consistent with the results of Western Blot). (2) Semi-quantitative RT-PCR results showed APP mRNA in the hippocampus of SAMP8,SAMR1 rats. There was only a slight increase in age, and the SAMP8 group was slightly higher than the SAMR1 group. But there was significant difference only in the 12 month old group. (3) Western Blot results showed that APP increased most significantly in 8 months old SAMP8 mice (p0. 01), and decreased at 12 months old. The results of 4) real time PCR showed that miR-101 decreased significantly in SAMP8 mice. (5) the change trend of mRNA and protein levels of COX-2 in each group was similar to that of APP, but there was no significant difference in mRNA and protein levels in COX-1. Conclusion: (1) the spatial memory disorder and A 尾 deposition can occur spontaneously in SAMP8 rats at early stage. (2) the expression of APP and COX-2 in hippocampus of SAMP8 rats is mainly regulated by posttranscriptional level. (3) miR-101 may regulate the expression of APP and COX-2 protein. (4) miR-101 may be a new drug target for the prevention and treatment of AD.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R749.16
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