核苷酸结合寡聚化结构域样受体蛋白3在阿尔茨海默病和血管性痴呆患者中的表达及相关因素研究

发布时间：2018-10-18 17:27

【摘要】：目的探讨核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)对痴呆发病的影响。方法 2014年1月~2015年10月,用RT-PCR方法检测16例阿尔茨海默病(AD)患者、22例血管性痴呆(VD)患者和20名年龄相似的健康体检者外周血单个核细胞中NLRP3 m RNA水平;ELISA法检测血清中白细胞介素(IL)-1β和IL-18蛋白水平;收集患者外周血白细胞计数(WBC)和血清总钙浓度等临床检测资料;将NLRP3 m RNA水平分别与IL-1β、IL-18、WBC和钙浓度进行单因素相关分析。结果 NLRP3 m RNA水平由高到低依次为AD组VD组健康组(q11.48,P0.05);IL-1β水平AD组高于健康组(q=16.74,P0.05),AD组与VD组、VD组与健康组无显著性差异(P0.05);IL-18水平各组间均无显著性差异(P0.05)。AD组中,NLRP3 m RNA水平与IL-1β水平(r=0.64)、血清钙水平(r=0.58)呈正相关,与IL-18、WBC无相关性(P0.05)。结论 NLRP3炎症小体中相关蛋白或基因表达与AD的发病可能有较大关系,与VD的发病关系不大。
[Abstract]:Objective to investigate the effect of nucleotide binding oligodeoxyribonucleotide domain like receptor protein 3 (NLRP3) on the pathogenesis of dementia. Methods from January 2014 to October 2015, The levels of NLRP3 m RNA in peripheral blood mononuclear cells (PBMC) of 16 patients with Alzheimer's disease (AD), 22 patients with vascular dementia (VD) and 20 healthy controls of similar age were detected by RT-PCR method, and the levels of interleukin (IL) 1 尾 and IL-18 protein were detected by ELISA assay. The clinical data of peripheral blood leukocyte count (WBC) and serum total calcium concentration (TTC) were collected, and the levels of NLRP3 m RNA were analyzed by univariate correlation with IL-1 尾, IL-18,WBC and Ca 2 + concentration, respectively. Results the level of NLRP3 m RNA in AD group was higher than that in healthy group (q11.48% P 0.05), IL-1 尾 level in AD group was higher than that in healthy group (qn16.74%), there was no significant difference between VD group and healthy group (P0.05), and there was no significant difference in IL-18 level among all groups (P0.05). AD group, NLRP3 RNA level). There was a positive correlation between the level of IL-1 尾 and serum calcium. There was no correlation with IL-18,WBC (P0.05). Conclusion the expression of related proteins or genes in NLRP3 inflammatory corpuscles may be related to the pathogenesis of AD, but not to the pathogenesis of VD.
【作者单位】：贵州医科大学第三附属医院医学实验中心;贵州医科大学第三附属医院神经内科;贵州医科大学第三附属医院精神科;贵州医科大学医学分子生物学重点实验室;
【基金】：国家自然科学基金项目(No.8156110223) 贵州省科技厅重大专项(No.黔科合计Z字2012-4010号)
【分类号】：R749.16

【相似文献】