重庆地区人感染博尔纳病病毒血清和分子流行病学初步研究

发布时间：2018-10-21 08:17

【摘要】：背景博尔纳病病毒（Borna disease virus，BDV）为非节段单股负链RNA病毒。该病毒无细胞毒性，且具有高度嗜神经细胞性并且可持续感染中枢神经系统。BDV的自然宿主种类范围广泛，据国内外报道BDV可自然或实验室感染马、兔、鼠、狗等多种温血类动物。动物感染该病毒后可表现出明显的精神、行为异常等神经精神症状。近年来大量国内外的流行病学研究关注人与BDV相互关系，其中许多研究结果均证实人类可能感染BDV。同时基于血清以及分子流行病学研究，BDV感染被认为与人类抑郁症等多种精神疾病发病相关。目的本研究选取重庆地区抑郁症患者以及健康人群作为研究对象。采用血清和分子流行病学研究技术，探讨BDV在重庆地区人群中流行病学规律，同时比较抑郁症患者与健康人群中BDV感染分布特点。方法 1.研究对象纳入：采用横断面研究方法，经过严格的研究对象纳入以及排除标准筛选，，本次研究共纳入914名研究对象。其中包括抑郁症入组534人（男性187人，女性347人）和健康对照入组380人（男性160人，女性220人）。 2.血清学检测：采用国外报道常用的ELISA方法检测血浆中BDV循环免疫复合物（CICs），每份血浆样本均重复测量两次，取两次OD值的平均值作为最终结果。 3.分子生物学检测：所有样本均进行分子生物学检测。使用全血标本提取RNA并逆转录获得相应的cDNA。其后使用荧光探针法RT-PCR检测样本中BDV p24片段。结果 1.血清学检测结果：本次检测共检出139例BDV循环免疫复合物呈不同程度阳性，总体阳性率为15.2%。其中健康对照组检出42份阳性血浆，阳性率为11.1%；在抑郁症组中检出97份阳性血浆，阳性率为18.2%。抑郁组研究对象BDV CICs阳性率明显高于健康对照组（X~2＝5.093，P=0.024）。 2.分子生物学检测结果：共检出35份阳性样本，总体阳性率为3.8%。在抑郁症组中共检出27份样本中含有p24片段，阳性率为5.1%；同时健康对照组中检出8份，阳性率为2.1%。抑郁组研究对象外周血p24检出率明显高于健康对照组（X~2＝5.250，P=0.022）。结论 1.本研究首次报道重庆地区抑郁症患者以及健康人群中人感染BDV的流行病学研究数据。 2.本研究血清学和分子生物学结果均提示在抑郁症患者中BDV感染率显著高于健康人群，与国外相关研究结果吻合。提示BDV感染可能与精神疾病相关。
[Abstract]:Background Borna disease virus (Borna disease virus,BDV) is a non-segmental single-stranded negative-stranded RNA virus. The virus is non-cytotoxic, highly neurophilic and sustainably infects the central nervous system. There are a wide range of natural hosts for BDV. It is reported at home and abroad that BDV can infect horses, rabbits, mice and mice naturally or in laboratory. Dogs and other warm-blooded animals. Animals infected with the virus can show obvious mental, behavioral disorders and other neuropsychiatric symptoms. In recent years, a large number of epidemiological studies at home and abroad have focused on the relationship between human beings and BDV, many of which have confirmed that humans may be infected with BDV.. At the same time, based on serological and molecular epidemiological studies, BDV infection is thought to be associated with various mental disorders such as depression in humans. Objective to study depression patients and healthy people in Chongqing. The epidemiological characteristics of BDV in Chongqing population were studied by using serum and molecular epidemiology techniques, and the distribution characteristics of BDV infection in depression patients and healthy people were compared at the same time. Method 1. Inclusion of study subjects: 914 subjects were included in this study through strict selection of inclusion and exclusion criteria using cross-sectional research method. These included 534 depression patients (187 men and 347 women) and 380 healthy controls (160 men and 220 women). Serological detection: the BDV circulating immune complex (CICs),) in plasma was detected by ELISA method reported abroad. The average value of OD in each plasma sample was measured twice and the average value of OD was taken as the final result. 3. Molecular biology detection: all samples were tested by molecular biology. RNA was extracted from whole blood samples and cDNA. was obtained by reverse transcription. The BDV p24 fragment was detected by fluorescence probe RT-PCR. Result 1. Serological results: a total of 139 cases of BDV circulating immune complex were positive in different degrees, and the overall positive rate was 15.22%. 42 positive plasma samples were detected in the healthy control group, the positive rate was 11.1, and 97 positive plasma samples were found in the depression group, the positive rate was 18.2%. The positive rate of BDV CICs in depression group was significantly higher than that in healthy control group (X2 + 5.093). Results of molecular biology: 35 positive samples were detected, the total positive rate was 3.8%. The positive rate of p24 fragment was 5.1 in 27 samples of depression group and 8 in healthy control group, the positive rate was 2.1%. The detection rate of p24 in peripheral blood in depression group was significantly higher than that in healthy control group (X2 + 5. 250 P + 0. 022). Conclusion 1. This study reported for the first time the epidemiological data of BDV infection among depression patients and healthy people in Chongqing. 2. The results of serology and molecular biology showed that the infection rate of BDV in depression patients was significantly higher than that in healthy people. The results suggest that BDV infection may be associated with mental illness.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R749.4

【参考文献】