加味不忘散对MicroRNA-30e海马过表达SD大鼠学习记忆及海马ChAT改变的影响

发布时间：2018-11-14 13:01

【摘要】：目的:本研究旨在探索MicroRNA-30e海马过表达SD大鼠模型认知功能障碍可能的发病机制及观察加味不忘散对该模型大鼠学习记忆功能的影响。方法:将40只健康雄性SD大鼠(200g±10g)随机分成5组,分别标记为空白对照组(简称B组)、空载体病毒对照组(简称C组)、MicroRNA-30e海马过表达模型组(简称M组)、加味不忘散低剂量组(简称L组)、加味不忘散高剂量组(简称H组)。给予M、L、H组大鼠双侧海马各注射MicroRNA-30e病毒颗粒1ul;给予C组大鼠双侧海马各注射空病毒颗粒1ul;给予B组大鼠双侧海马各注射生理盐水1ul。术后2周,H、L组分别给予高(0.3g/100g体重)、低(0.15g/100g体重)剂量加味不忘散溶液灌胃,其他三组给以生理盐水(1ml/100g体重)灌胃。持续2周后,采用Morris水迷宫实验评价大鼠学习记忆功能。Morris水迷宫实验完成后将所有大鼠断头取脑,分离双侧海马组织,制备组织匀浆,利用比色法检测各样本ChAT活力的改变。结果:1.Morris水迷宫实验结果:与B组比较,C组各指标(每天逃避潜伏期、跨台次数及原平台象限停留时间)变化均无显著差异(P0.05);与B、C组比较,M组逃避潜伏期在第2-5天显著延长(p0.05),跨台次数显著减少(p0.05),原平台象限停留时间显著缩短(p0.05);与M组比较,L、H组逃避潜伏期在第2-5天显著缩短(p0.05),跨台次数显著增加(p0.05),原平台象限停留时间显著延长(p0.05);与L组比较,H组逃避潜伏期在第2-5天显著缩短(p0.05),跨台次数显著增加(p0.05),原平台象限停留时间显著延长(p0.05)。2.ChAT活力测定结果:与B组比较,C组海马ChAT活力变化无显著差异(P0.05);与B、C组比较,M组海马ChAT活力显著降低(p0.05);与M组比较,H、L组海马ChAT活力均显著升高(p0.05);与L组比较,H组海马ChAT活力显著升高(p0.05)。结论:1.加味不忘散可显著改善MicroRNA-30e海马过表达SD大鼠学习记忆功能,且高、低剂量之间具有浓度依赖性。2.MicroRNA-30e海马过表达致SD大鼠认知功能障碍的机制与大鼠海马ChAT活力降低有关,加味不忘散可提高模型大鼠海马ChAT活力,且高、低剂量之间具有浓度依赖性。
[Abstract]:Aim: to explore the possible pathogenesis of cognitive dysfunction in MicroRNA-30e hippocampus overexpression SD rat model and to observe the effect of Jiawei Fujisan on learning and memory function in this model. Methods: forty healthy male SD rats (200g 卤10g) were randomly divided into 5 groups: blank control group (group B), empty carrier virus control group (group C) and MicroRNA-30e hippocampal overexpression model group (M group). The low dose group (L group) and the high dose group (H group) were added. MicroRNA-30e virus particles were injected into both hippocampus of rats in group C, empty virus particles were injected into hippocampus of rats in group C, and normal saline was injected into hippocampus of rats in group B. Two weeks after the operation, the patients in group L were treated with high (0.3g/100g body weight) and low dose (0.15g/100g weight), respectively. The other three groups were treated with normal saline (1ml/100g body weight). After 2 weeks, Morris water maze test was used to evaluate the learning and memory function of rats. After the Morris water maze experiment, all the rats were cut off their brains, the bilateral hippocampal tissues were isolated, and the tissue homogenate was prepared. The changes of ChAT activity in each sample were detected by colorimetric method. Results: the results of 1.Morris water maze test showed that there was no significant difference between group C and group B (P 0.05). Compared with group C, the escape latency of group M was significantly prolonged (p0.05), the number of transmissions was significantly decreased (p0.05), and the residence time of the original platform quadrant was significantly shortened (p0.05). Compared with group M, the escape latency of LHH group was significantly shortened (p0.05), the number of transmissions was significantly increased (p0.05), and the residence time of the original platform quadrant was significantly prolonged (p0.05). Compared with group L, the escape latency of group H was significantly shorter than that of group L (p0.05), and the number of transmissions was significantly increased (p0.05). The residence time of the original platform quadrant was significantly prolonged (p0.05). The results of 2.ChAT activity: compared with group B, there was no significant difference in ChAT activity in hippocampus between group C and group B (P0.05). Compared with group C, the activity of ChAT in hippocampus of group M was significantly lower than that of group B (p0.05), the activity of ChAT in hippocampus of group H was significantly higher than that of group M (p0.05), and the activity of ChAT in hippocampus of group H was significantly higher than that of group L (p0.05). Conclusion: 1. Jiawei Fujisan could significantly improve the learning and memory function of SD rats over-expressed in MicroRNA-30e hippocampus. The mechanism of cognitive dysfunction in SD rats induced by over-expression of 2.MicroRNA-30e hippocampus was related to the decrease of ChAT activity in hippocampus of rats. Jiawei did not forget powder could increase the ChAT activity in hippocampus of model rats. There was a concentration-dependent relationship between low doses.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R749.1

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