外源性硫化氢对血管性痴呆模型大鼠学习记忆的影响

发布时间：2018-12-11 13:23

【摘要】：目的观察外源性硫化氢(Hydrogen Sulfide,H2S)对血管性痴呆大鼠(Vascular Dementia,Va D)学习记忆改善情况及血管性痴呆大鼠血浆硫化氢含量的变化。方法采用改良的四血管法(4-VO)复制大鼠Va D模型,将模型大鼠按给药干预时间分为1 d组、7 d组、30 d组,每组分为假手术组、模型组、低剂量组、高剂量组。低剂量组每日腹腔注射H2S供体Na HS 30μmol/kg,高剂量组每日腹腔注射H2S供体Na HS 100μmol/kg,假手术组与模型组每日腹腔注射等量生理盐水,然后进行Morris水迷宫检测大鼠学习记忆能力,用HE染色观察海马神经元的损伤情况,并测定Va D大鼠模型血浆硫化氢含量。结果与假手术组相比,模型组逃避潜伏期明显增长(P0.05),血浆硫化氢浓度明显减少(P0.05),海马神经元损伤严重;与模型组相比,低剂量组与高剂量组逃避潜伏期明显缩短(P0.05),血浆硫化氢浓度明显增加(P0.05),海马神经元损伤减轻,血管性痴呆大鼠学习记忆有所改善。结论硫氢化钠可改善Va D大鼠的认知水平和病理变化,其保护作用可能与提高硫化氢含量有关。
[Abstract]:Objective to observe the effects of exogenous hydrogen sulfide (Hydrogen Sulfide,H2S) on learning and memory in vascular dementia rats (Vascular Dementia,Va D) and the changes of plasma hydrogen sulfide content in vascular dementia rats. Methods the rat model of Va D was induced by modified four-vessel method (4-VO). The model rats were divided into 1 day group, 7 day group and 30 day group according to the time of intervention. Each group was divided into sham operation group, model group, low dose group and high dose group. H2S donor Na HS 30 渭 mol/kg, was injected intraperitoneally every day in low dose group and Na HS 100 渭 mol/kg, model group and model group were injected intraperitoneally with the same amount of normal saline daily. Then Morris water maze was used to detect the learning and memory ability of rats, the damage of hippocampal neurons was observed by HE staining, and the content of hydrogen sulfide in plasma of Va D rats was measured. Results compared with sham operation group, the escape latency of model group was significantly increased (P0.05), plasma hydrogen sulfide concentration was significantly decreased (P0.05), hippocampal neuron injury was serious. Compared with the model group, the escape latency of low-dose group and high-dose group was significantly shortened (P0.05), plasma hydrogen sulfide concentration significantly increased (P0.05), hippocampal neuron injury was alleviated, learning and memory of vascular dementia rats improved. Conclusion Sodium sulphide can improve the cognitive and pathological changes of Va D rats, and its protective effect may be related to the increase of hydrogen sulfide content.
【作者单位】：贵阳医学院分子生物学重点实验室;贵州省人民医院;
【基金】：国家自然科学基金(81160149) 贵州省科技厅社会攻关计划项目(黔科合SY[2010]3076)
【分类号】：R-332;R749.13

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