吗啡急性戒断大鼠边缘前皮层无线遥测脑电活动的研究

发布时间：2019-01-25 21:19

【摘要】：目的探讨吗啡急性戒断大鼠边缘前皮层(prelimbic cortex,PrL)脑电活动在吗啡成瘾机制中的作用。方法将大鼠随机分为吗啡实验组和生理盐水对照组。进行脑立体定位电极埋藏手术,建立吗啡依赖大鼠条件性位置偏爱(conditioned place preference,CPP)模型。测试各组大鼠的CPP行为,同时遥测分析不同行为状态下PrL的脑电活动。结果实验组大鼠戒断d 1、d 3,在白箱内停留时间明显延长(组间、组内比较)。与对照组相比,实验组大鼠戒断d 3,在黑白箱停留及黑-白箱穿梭时,PrL脑电β波明显增加,δ波明显减少;当白-黑箱穿梭时,β波明显减少,δ波明显增加;α波及θ波在各种行为状态下,组间比较差异均无显著性。结论吗啡急性戒断大鼠觅药行为的产生伴随着PrL脑电β波及δ波的特异性改变,提示PrL脑电改变可能与吗啡依赖大鼠觅药动机形成有关。
[Abstract]:Objective to investigate the role of brain electrical activity in the prelimbic cortex (prelimbic cortex,PrL) of morphine abstinent rats. Methods Rats were randomly divided into morphine experimental group and saline control group. The model of conditioned place preference (conditioned place preference,CPP) in morphine dependent rats was established by stereotactic electrode implantation. The CPP behavior of each group of rats was measured and the EEG activity of PrL in different behavioral states was analyzed by telemetering. Results the rats in the experimental group had a longer stay in the white box on 1 and 3 days after abstinence (inter-group, intra-group comparison). Compared with the control group, the 尾 wave and 未 wave of PrL in the black-and-white box and black and white box were significantly increased, and the 尾 wave and 未 wave were obviously decreased when the white and black box shuttled in the experimental group on the 3rd day after abstinence, and the 尾 wave and 未 wave were obviously increased when the white and black box shuttled through the box. There was no significant difference in 伪 wave and 胃 wave between the two groups. Conclusion the occurrence of drug seeking behavior in acute morphine withdrawal rats is accompanied by the specific changes of PrL 尾 and 未 waves, suggesting that the changes of PrL EEG may be related to the formation of drug seeking motivation in morphine dependent rats.
【作者单位】：皖南医学院生理学教研室;皖南医学院医学影像与检验学院;皖南医学院形态实验中心;
【基金】：安徽省自然科学基金资助项目(No 090413096) 皖南医学院中青年科研基金资助项目(No WK201204)
【分类号】：R749.61

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