雌激素对抑郁症小鼠行为学和脑组织单胺类递质的作用

发布时间：2019-01-29 04:45

【摘要】：目的:探讨雌激素低下对抑郁症模型小鼠行为学影响及神经生化机制。方法:清洁级昆明小鼠随机分为假手术组(Sham)、卵巢切除组(OVX)、慢性应激组(CUMS)、卵巢切除+慢性应激组(OVX+CUMS)和雌激素治疗组(E组,0.15 mg/kg),每组10只。给药组于每日应激前1 h,ig给药,其余各组给予等体积生理盐水,共计21 d。采用卵巢切除法(OVX)制备雌激素低下动物模型,采用CUMS法制备抑郁症小鼠模型。观察各组小鼠一般体征,行阴道上皮角化实验,通过旷场实验(OFT)、强迫游泳实验(FST)及体重测试(BWM)观察其行为学变化,采用ELISA法检测脑组织单胺类递质五羟色胺(5-HT)及多巴胺(DA)含量变化。结果:OVX小鼠,阴道细胞涂片未见动情周期变化连续7 d。模型小鼠在第21 d,与Sham组比较,(1)OVX和CUMS小鼠体重增加(P㩳0.01),而OVX+CUMS则体重下降(P㩳0.01);(2)在OVX和CUMS小鼠,OFT水平和垂直运动得分减少(P㩳0.01),而在E组小鼠,OFT水平和垂直运动得分增加(P㩳0.05);(3)在OVX和CUMS小鼠,FST不动时间减少(P㩳0.01),但在E组小鼠,FST不动时间增加(P㩳0.01)。(4)在OVX组小鼠,阴道脱落细胞呈现大量白细胞和少量上皮细胞。在CUMS和OVX小鼠,脑组织5-HT和DA含量减少(P㩳0.01),但在E组小鼠,脑组织中5-HT、DA含量增加(P㩳0.05,P㩳0.01)。结论:小鼠抑郁行为可以由于雌激素低下诱发,并且与脑组织5-HT和DA含量下调有关。补充雌激素可能通过增加单胺类递质5-HT和DA含量在脑组织,从而改善小鼠抑郁行为。
[Abstract]:Objective: to investigate the effect of low estrogen on behavior and neurobiochemical mechanism of depression model mice. Methods: Kunming mice of clean grade were randomly divided into sham-operated group, (Sham), ovariectomized group, (OVX), chronic stress group, (CUMS), ovariectomized chronic stress group, (OVX CUMS) group and estrogen treatment group (E group, 0.15 mg/kg). 10 rats in each group. The rats in the administration group were given intravenously 1 hour before daily stress, and the other groups were given the same volume of normal saline for 21 days. The ovariectomized (OVX) was used to establish the animal model of estrogen hypoestrogen and the CUMS method was used to establish the depression model in mice. The general signs of each group were observed, the vaginal epithelialization test was performed, the behavioral changes were observed by (OFT), forced swimming test (FST) and body weight test (BWM). The changes of monoamine transmitters (5-HT) and dopamine (DA) (DA (DA) in brain tissue were detected by ELISA method. Results: the vaginal cell smear of OVX mice did not show estrous cycle change for 7 days. On the 21st day, compared with the Sham group, the model mice (1) OVX and CUMS mice gained weight (P0. 01), while the OVX CUMS mice lost weight (P0. 01). (2) in OVX and CUMS mice, the scores of OFT level and vertical movement decreased (P0. 01), while in group E, OFT level and vertical movement scores increased (P0. 05). (3) in OVX and CUMS mice, FST immobility time decreased (P0. 01), but in E group, FST immobility time increased (P0. 01). (4) in OVX group, vaginal exfoliated cells showed a large number of white blood cells and a small number of epithelial cells. In CUMS and OVX mice, the contents of 5-HT and DA in brain tissue decreased (P0. 01), but in group E, the content of 5-HTN-DA in brain tissue increased (P0. 05% P0. 01). Conclusion: depression can be induced by low estrogen in mice, and may be related to the down-regulation of 5-HT and DA in brain tissue. Estrogen supplementation may improve the depressive behavior of mice by increasing the contents of monoamine transmitters 5-HT and DA in brain tissue.
【作者单位】：解放军第323医院普通外科;第四军医大学唐都医院急诊科;解放军兰州总医院干部病房;解放军第451医院神经内科;
【基金】：全军医药卫生科研基金(CLZ11JB02) 甘肃省自然科学基金(120RJZA274)
【分类号】：R749.4

【相似文献】