β片层阻断肽H102对PAP小鼠脑内ERK信号转导通路的影响
发布时间：2019-02-25 19:35
【摘要】：目的研究β片层阻断肽H102对PAP双转基因小鼠脑内ERK信号转导通路的激活作用。方法将PAP双转基因小鼠随机分为模型组和给药组,每组10只,设同背景C57BL/6J小鼠为对照组。给药组每日鼻腔给予H102溶液(5.8 mg/kg)5μL,对照组、模型组每日鼻腔给予等体积H102空白辅料溶液。30 d后行Morris水迷宫测试。之后采用免疫组化及免疫印迹技术观察小鼠脑组织内RAS、P-MEK及P-ERK蛋白的表达变化。结果 (1)Morris水迷宫测试。模型组小鼠学习记忆能力较对照组显著降低,给药组较模型组显著提高(均P0.05)。(2)免疫组化及免疫印迹检测结果。模型组脑内RAS、P-MEK及P-ERK表达较对照组显著降低,给药组蛋白表达较模型组显著增高(均P0.01)。结论β片层阻断肽H102可激活PAP双转基因小鼠脑内ERK信号转导通路,增加神经细胞PAS、P-MEK及P-ERK的含量,改善PAP小鼠学习记忆能力。
[Abstract]:Aim to study the effect of 尾-lamellar blocking peptide H102 on the activation of ERK signal transduction pathway in the brain of PAP double transgenic mice. Methods PAP double transgenic mice were randomly divided into two groups: model group (n = 10) and administration group (n = 10). C57BL/6J mice with the same background were used as control group. The rats in the treatment group were given 5 渭 L H102 solution (5.8 mg/kg) per day, and the control group and the model group were given the same volume of H102 blank adjunct solution every day. After 30 days, the Morris water maze test was performed. Immunohistochemical and immunoblotting techniques were used to observe the expression of RAS,P-MEK and P-ERK protein in the brain tissue of mice. Results (1) Morris water maze test. The learning and memory ability of the model group was significantly lower than that of the control group, and the results of immunohistochemistry and immunoblotting were significantly improved in the administration group than in the model group (P0.05). (2). The expression of RAS,P-MEK and P-ERK in the brain of the model group was significantly lower than that of the control group, and the expression of the protein in the administration group was significantly higher than that of the model group (P0.01). Conclusion 尾-lamellar blocking peptide H102 can activate the ERK signal transduction pathway in the brain of PAP double transgenic mice, increase the contents of PAS,P-MEK and P-ERK in nerve cells, and improve the learning and memory ability of PAP mice.
【作者单位】： 天津医科大学生理教研室;天津医科大学内分泌研究所;
【基金】：国家科技重大专项基金资助项目(2009ZX09103029) 天津市科技支撑重点项目(09ZCKFSH00100)
【分类号】：R749.16
，
本文编号：2430468
[Abstract]:Aim to study the effect of 尾-lamellar blocking peptide H102 on the activation of ERK signal transduction pathway in the brain of PAP double transgenic mice. Methods PAP double transgenic mice were randomly divided into two groups: model group (n = 10) and administration group (n = 10). C57BL/6J mice with the same background were used as control group. The rats in the treatment group were given 5 渭 L H102 solution (5.8 mg/kg) per day, and the control group and the model group were given the same volume of H102 blank adjunct solution every day. After 30 days, the Morris water maze test was performed. Immunohistochemical and immunoblotting techniques were used to observe the expression of RAS,P-MEK and P-ERK protein in the brain tissue of mice. Results (1) Morris water maze test. The learning and memory ability of the model group was significantly lower than that of the control group, and the results of immunohistochemistry and immunoblotting were significantly improved in the administration group than in the model group (P0.05). (2). The expression of RAS,P-MEK and P-ERK in the brain of the model group was significantly lower than that of the control group, and the expression of the protein in the administration group was significantly higher than that of the model group (P0.01). Conclusion 尾-lamellar blocking peptide H102 can activate the ERK signal transduction pathway in the brain of PAP double transgenic mice, increase the contents of PAS,P-MEK and P-ERK in nerve cells, and improve the learning and memory ability of PAP mice.
【作者单位】： 天津医科大学生理教研室;天津医科大学内分泌研究所;
【基金】：国家科技重大专项基金资助项目(2009ZX09103029) 天津市科技支撑重点项目(09ZCKFSH00100)
【分类号】：R749.16
，
本文编号：2430468
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