迟发型阿尔茨海默病与NEDD9基因rs760678位点多态性的相关性研究
发布时间：2019-05-09 19:54
【摘要】：[目的]探讨中国云南汉族人群中NEDD9基因rs760678位点单核苷酸多态性与迟发型阿尔茨海默病、轻度认知功能障碍的相关性。 [方法]分别随机入组云南汉族93例LOAD组患者,男性42例,女性51例,平均年龄75.1±6.4岁；67例MCI组患者,男性37例,女性30例,平均年龄73.7±5.5岁；101例正常对照者,男性55例,女性46例,平均年龄72.6±5.6岁,采用聚合酶链式反应(PCR)技术及限制性内切酶技术对所有研究对象的NEDD9基因rs760678位点突变进行分型,采用spssl7.0统计软件进行统计分析,采用t检验、χ2检验、方差分析的方法,比较分析三组间基因型频率、等位基因频率与年龄、性别以及LOAD组疾病严重程度的相关性；分析比较LOAD组、MCI组不同基因型、性别与MMSE量表各项评分的关联。所有统计检验均以P0.05为差异有统计学意义。计数资料用%表示,计量资料用均数±标准差(x±s)表示。 [结果]LOAD组、MCI组和正常对照组三组受试者基因型和等位基因频率分布均符合Hardy-Weinberg遗传平衡定律,差异无统计学意义(P=0.889,P=0.648)进一步按性别分层后三组基因型之间差异无统计学意义(P0.05),LOAD组、MCI组MMSE量表各项评分与基因型、性别之间差异无统计学意义(P0.05)。LOAD组按照疾病严重程度进行分层后基因型间差异间无统计学意义(P0.05)。 [结论]本研究在中国云南汉族人群中,NEDD9基因rs760678位点单核苷酸多态性与迟发型阿尔茨海默病、轻度认知功能障碍患者的发病无明显关联。
[Abstract]:[objective] to investigate the relationship between rs760678 polymorphism of NEDD9 gene and delayed Alzheimer's disease (AD) and mild cognitive impairment in Yunnan Han population. [methods] 93 patients with LOAD in Yunnan Han nationality were randomly enrolled, including 42 males and 51 females, with an average age of 75.1 卤6.4 years, 67 patients in MCI group, 37 males and 30 females, with an average age of 73.7 卤5.5 years. There were 55 males and 46 females with an average age of 72.6 卤5.6 years. Polymerase chain reaction (PCR) technique and restriction endonuclease technique were used to type the rs760678 mutation of NEDD9 gene in all the subjects. Spssl7.0 software was used for statistical analysis. T test, 蠂 2 test and variance analysis were used to compare and analyze the correlation between genotype frequency, allele frequency and age, sex and severity of disease in LOAD group. The relationship between different genotypes, sex and MMSE scores in LOAD group and MCI group was analyzed and compared. All statistical tests were statistically significant (P 0.05). The counting data are represented by%, and the measurement data are represented by mean 卤standard deviation (x 卤s). [results] the distribution of genotype and allele frequency in LOAD group, MCI group and normal control group were in accordance with the law of Hardy-Weinberg genetic balance, and the difference was not statistically significant (P 鈮，
本文编号：2473040
[Abstract]:[objective] to investigate the relationship between rs760678 polymorphism of NEDD9 gene and delayed Alzheimer's disease (AD) and mild cognitive impairment in Yunnan Han population. [methods] 93 patients with LOAD in Yunnan Han nationality were randomly enrolled, including 42 males and 51 females, with an average age of 75.1 卤6.4 years, 67 patients in MCI group, 37 males and 30 females, with an average age of 73.7 卤5.5 years. There were 55 males and 46 females with an average age of 72.6 卤5.6 years. Polymerase chain reaction (PCR) technique and restriction endonuclease technique were used to type the rs760678 mutation of NEDD9 gene in all the subjects. Spssl7.0 software was used for statistical analysis. T test, 蠂 2 test and variance analysis were used to compare and analyze the correlation between genotype frequency, allele frequency and age, sex and severity of disease in LOAD group. The relationship between different genotypes, sex and MMSE scores in LOAD group and MCI group was analyzed and compared. All statistical tests were statistically significant (P 0.05). The counting data are represented by%, and the measurement data are represented by mean 卤standard deviation (x 卤s). [results] the distribution of genotype and allele frequency in LOAD group, MCI group and normal control group were in accordance with the law of Hardy-Weinberg genetic balance, and the difference was not statistically significant (P 鈮，
本文编号：2473040
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