T细胞活化信号分子基因miRNA结合区域SNP与乙肝疫苗弱应答的关联研究

发布时间：2018-01-09 15:20

本文关键词：T细胞活化信号分子基因miRNA结合区域SNP与乙肝疫苗弱应答的关联研究　出处：《现代免疫学》2015年03期 　论文类型：期刊论文

【摘要】：探讨与T细胞激活相关基因miRNA结合区域单核苷酸多态性(SNP)与乙肝疫苗弱应答的关联。采用病例对照设计,检测比较与T细胞激活相关基因miRNA结合区域SNP在乙肝疫苗正常应答组与弱应答组分布情况,及对乙肝疫苗抗体水平影响。经生物信息学预测,从T细胞激活相关的93个基因,发现30个相关基因miRNA结合区域有63个SNP,其中,有37个SNP位于11个基因的种子区,在中国人群最小等位基因频率(MAF)10%的有20个。根据野生型等位基因和相应的突变型与miRNA结合自由能的改变情况≥2.80KJ/mol筛选出6个SNP作本研究备选SNP(rs1131579,rs17548629,rs12609318,rs344561,rs15927,rs3804033),rs12609318因为不符合HWE平衡而排除,比较77名弱应答者和207名正常应答者上述5个SNP基因型与等位基因型分布情况,差异无统计学意义(P0.05),正常应答者的乙肝疫苗抗体滴度与单核苷酸多态性之间亦无关联(P0.05)。本研究未能证明T细胞激活相关基因miRNA结合区域SNP与乙肝疫苗接种弱应答发生存在关联。
[Abstract]:Discuss with T cell activation related gene miRNA with single nucleotide polymorphisms (SNP) and hepatitis B vaccine hyporesponse Association. A case-control design, compared with the detection of T cell activation related gene miRNA SNP binding region of hepatitis B vaccine in normal response group and low response group distribution, and the influence of the antibody level of hepatitis B vaccine by biological. It was predicted, activation of 93 genes from T cells, found 30 genes related to miRNA with 63 SNP, the seed region, 37 SNP in 11 genes, in China population the minimum allele frequency (MAF) 10% 20. According to the wild type. Gene and mutant miRNA and corresponding binding free energy change was screened by 2.80KJ/mol 6 SNP for the study of SNP (rs1131579, rs17548629, alternative rs12609318, rs344561, rs15927, rs3804033, rs12609318) for row does not conform to the HWE balance In addition, the distribution of 77 weak responders and 207 normal responders above 5 SNP genotype and allele, there was no statistically significant difference (P0.05), between normal responders to hepatitis B vaccine antibody titer and single nucleotide polymorphism has no association (P0.05). This study did not prove that T cell activation related gene miRNA binding region of SNP and hepatitis B vaccination hyporesponse are connected.

【作者单位】：广东医学院校医务室;广东医学院公共卫生学院;
【基金】：广东省高等学校学科与专业建设专项资金(2013KJCX0091) 湛江市科技计划项目(2013B01130) 广东医学院科研基金(XK1429)
【分类号】：R186
【正文快照】： 乙型肝炎(简称乙肝)是当今世界一个严重危害人类健康和生命安全的公共卫生问题,据世界卫生组织(WHO)报道,全球约20亿人曾感染过HBV,其中3.5亿人为慢性HBV感染者,每年约有100万人死于HBV感染所致的肝衰竭、肝硬化和原发性肝细胞癌(HHC)[1]。我国是HBV感染的高发区,乙肝的防治刻

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