GFP标记的重组腺病毒在消毒效果评价中的应用研究

发布时间：2018-02-28 16:33

本文关键词： 重组腺病毒荧光计数复制缺陷病毒灭活试验　出处：《中国消毒学杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的研究GFP标记的复制缺陷型腺病毒在消毒剂灭活病毒试验中应用的可行性。方法采用商品化Ad-easy系统经同源重组包装GFP标记的复制缺陷型腺病毒,扩增后进行荧光计数和复制缺陷性能检测,其作为指示病毒进行病毒灭活试验。结果腺病毒基因同源重组后转染AD-293细胞出现绿色荧光和细胞病变,成功包装出病毒颗粒,扩增后经荧光计数病毒滴度为4.20×10~6gfu/ml。盲传3代后,感染Vero细胞未出现荧光和细胞病变,表明无子代病毒的产生。以有效氯含量200 mg/L消毒剂作用于腺病毒20 min后,杀灭对数值为4.10。结论荧光标记的复制缺陷型腺病毒在含氯消毒剂对病毒杀灭能力检测评价中具有一定的可行性,更为高效和安全。
[Abstract]:Objective to study the feasibility of replication-deficient adenovirus labeled with GFP in disinfectant inactivation test. Methods Replication-deficient adenovirus labeled by GFP was packaged with commercial Ad-easy system. The results showed that the adenovirus gene homologous recombination showed green fluorescence and cytopathic changes in AD-293 cells, and the virus particles were successfully packaged. After amplification, the virus titer was 4.20 脳 10 ~ (-1) 6g / ml. After 3 generations of blind transmission, there was no fluorescence and cytopathic changes in infected Vero cells, indicating that no offspring virus was produced. The virus was treated with 200 mg/L disinfectant with available chlorine content for 20 min. Conclusion the fluorescent labeled replication-deficient adenovirus is feasible and more efficient and safe in the detection and evaluation of chlorinated disinfectant's ability to kill the virus.
【作者单位】：北京市疾病预防控制中心北京市预防医学研究中心;
【基金】：国家自然科学基金(8150081059) 首都卫生发展科研专项项目(首发2016-4-3012) 北京市预防医学研究中心科研培育专项(2015-BJYJ-14)
【分类号】：R187

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