内蒙古、新疆地区恙虫病流行病学调查研究

发布时间：2018-03-12 06:36

本文选题：恙虫病东方体　切入点：啮齿动物　出处：《中国人民解放军军事医学科学院》2007年硕士论文　论文类型：学位论文

【摘要】： 恙虫病是经恙螨叮咬传播的一种自然疫源性疾病，其病原体为恙虫病东方体(Orientia tsutsugamushi，Ot)。长期以来，恙虫病仅在我国长江以南广大地区流行，自1986年在长江以北的山东、江苏地区发现恙虫病流行后，疫区不断向北、向西扩展，严重威胁人民群众健康。然而内蒙古地区至今尚无该病报道，新疆仅有一篇Ot血清学调查报告。本研究的目的是调查内蒙古、新疆地区是否存在Ot自然感染，并探讨这两个地区Ot与其他地区Ot基因序列之间的变异和亲缘关系。在内蒙古、新疆地区选点，应用间接免疫荧光法(IFA)检测Ot-IgG抗体，结果显示两个地区人群血清Ot-IgG抗体阳性率分别为11.11％(13／117)和11.56％(34／294)。多因素Logistic回归分析结果显示，男性感染Ot的相对危险度高于女性(OR=7.150)，乌鲁木齐市乌拉泊三队感染Ot的相对危险度高于米泉市三道坝镇(OR=9.698)。采用巢式PCR方法对内蒙古、新疆地区鼠类脾脏进行检测，，Ot感染率分别为3.57％和2.50％；在内蒙古收集的168只鼠中，黑线姬鼠占34.5％，且有2只鼠Ot-Sta56基因扩增阳性，提示黑线姬鼠为当地优势带菌鼠；恙螨调查显示，内蒙古地区鼠类带螨率为6.82％，恙螨指数为0.49，在收集到的108只恙螨中，居中纤恙螨104只，无前恙螨属恙螨4只，提示当地9月份鼠体外以居中纤恙螨为优势螨种。将内蒙古、新疆地区扩增阳性标本的Ot-Sta56基因序列与收集到的其他地区Ot进行比较，结果显示内蒙古N59、N69、新疆X33及广东、东北分离株属Karp型；内蒙古N90属Oishi型；山东分离株和病人焦痂属Kawasaki型；内蒙古N65、N88处于独立的分支上。另外，对N65 Ot-Sta56全基因序列分析结果进一步支持了上述结论。提示N65 Ot-Sta56基因序列发生了较大变异。本研究证实内蒙古、新疆部分地区人群、鼠类中存在Ot自然感染，并且内蒙古地区部分Ot-Sta56基因序列发生较大变异。
[Abstract]:Tsutsugamushi typhus is a natural disease transmitted by chigger mites. Its pathogen is Orientia tsugamushius Otchus. For a long time, tsutsugamus tsutsugamushius has been prevalent only in large areas south of the Yangtze River in China. Since 1986, tsutsugamus tsutsugamus has been prevalent in Shandong, north of the Yangtze River. After the epidemic of tsutsugamushi disease was discovered in Jiangsu, the epidemic area continued to expand northward and westward, seriously threatening the health of the people. However, there has been no report of the disease in Inner Mongolia so far. The purpose of this study was to investigate whether there is any natural infection of Ot in Inner Mongolia and Xinjiang, and to explore the variation and genetic relationship between the Ot gene sequences of these two regions and other regions. In Inner Mongolia and Xinjiang, indirect immunofluorescence assay was used to detect Ot-IgG antibody. The results showed that the positive rates of serum Ot-IgG antibody in the two regions were 11.1113 / 117) and 11.56% / 294% respectively. The results of multivariate Logistic regression analysis showed that. The relative risk of infection of Ot in males was higher than that in females, and the relative risk of infection of Ot in three teams of Wulapo in Urumqi was higher than that in Oran 9.698in Sandaoba town of Miquan. The infection rate of Ot was 3.57% and 2.50 respectively in the spleen of mice in Inner Mongolia and Xinjiang by nested PCR method, 34.5% of them were Apodemus agrarius collected in Inner Mongolia, and 2 of them were positive for Ot-Sta56 gene amplification. The results indicated that Apodemus agrarius was the dominant local carrying mite, and the rate of carrying mites was 6.82and the index of chigger mites was 0.49 in Inner Mongolia. Among 108 chigger mites collected, 104 were intermediate chigger mites, 4 were chigger mites without the genus chigger mites. It suggested that in September, the dominant mites were chigger mites. The Ot-Sta56 gene sequences of amplified positive samples in Inner Mongolia and Xinjiang were compared with those in other regions. The results showed that N59 N69, X33 and Guangdong in Inner Mongolia belonged to Karp type, N90 belonged to Oishi type in Inner Mongolia. Shandong isolate and patient scab belong to Kawasaki type, and N65 Ot-Sta56 N88 is an independent branch in Inner Mongolia. In addition, the analysis of N65 Ot-Sta56 gene sequence further supports the above conclusion, which suggests that the N65 Ot-Sta56 gene sequence has changed greatly. In this study, it was confirmed that there was a natural infection of ant in population and rodents in Inner Mongolia and Xinjiang, and some Ot-Sta56 gene sequences were changed greatly in Inner Mongolia.
【学位授予单位】：中国人民解放军军事医学科学院
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R181.3;R513.2

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