2011~2012年河北省急性弛缓性麻痹病例中非脊髓灰质炎肠道病毒血清型的分布

发布时间：2018-03-13 06:23

本文选题：河北省　切入点：急性弛缓性麻痹　出处：《病毒学报》2014年01期 　论文类型：期刊论文

【摘要】：为了解2011~2012年河北省急性弛缓性麻痹(Acute flaccid paralysis,AFP)病例中非脊髓灰质炎肠道病毒(Non-polio enterovirus,NPEV)的血清型分布,探讨这些病毒与AFP的关系,本研究按照世界卫生组织要求,对河北省AFP病例粪便标本利用人横纹肌肉瘤(Human rhabdomyosarcoma,RD)细胞和转人脊髓灰质炎病毒受体的小鼠肺(Mouse cell line expressing the gene for the human cellular receptor for poliovirus,L20B)细胞进行病毒分离。筛检出NPEV毒株后,进行VP1区核苷酸序列测定,使用分子定型方法对NPEV进行血清型别鉴定。结果显示,在2011~2012年AFP病例中分离到的82株NPEV中共鉴定出A组人肠道病毒(HEV-A)42株(55.3%),包含4个血清型;B组人肠道病毒(HEV-B)34株(44.7%),包含13个血清型别,未鉴定出C组和D组人肠道病毒。另外还有2株腺病毒,4株未定型NPEV。HEV-A组中又以肠道病毒71型(EV-A71)为主要型别,在HEV-A中比例高达85.7%。2011~2012年河北省AFP病例中分离到的NPEV以HEV-A居多,尤其是EV-A71,为AFP病例中的优势毒株。
[Abstract]:In order to understand the serotype distribution of non poliomyelitis enterovirusNPEV (Non-polio enterovirusNPEV) in acute flaccid paralysis flaccid paralysissis (AFP) cases in Hebei Province from January to 2012, and to explore the relationship between these viruses and AFP, the present study was conducted in accordance with the requirements of the World Health Organization (WHO). Human rhabdomyosarcomavirus cells and mouse lung cell line expressing the gene for the human cellular receptor for poliovirusL20B cells were isolated from fecal specimens of AFP cases in Hebei Province. The nucleotide sequence of VP1 region was determined and the serotype of NPEV was identified by molecular typing method. A total of 82 strains of NPEV isolated from AFP cases from January to 2012 identified 42 strains of HEV-AV in group A, including 4 serotypes of human enterovirus group B, 34 strains of human enterovirus, including 13 serotypes. In addition, there were 2 strains of adenovirus in group C and D, in which the main type was Enterovirus 71 EV-A71. the proportion of NPEV in HEV-A was as high as 85.7%. From 2012 to 2012, most of the NPEV isolated from AFP cases in Hebei Province were HEV-A. EV-A 71, in particular, is the dominant strain in AFP cases.
【作者单位】：河北省疾病预防控制中心;
【分类号】：R181.3

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