首例由拥挤棒状杆菌引起白喉样病例的调查分析

发布时间：2018-03-16 17:47

本文选题：拥挤棒状杆菌　切入点：白喉样病例　出处：《中国疫苗和免疫》2017年01期 　论文类型：期刊论文

【摘要】：目的对一例由拥挤棒状杆菌引起的白喉样病例进行流行病学调查和病原学分析,为临床鉴别诊断和治疗提供依据。方法对白喉样病例及其密切接触者进行流行病学调查,并采集咽部病灶拭子标本进行细菌分离、培养和鉴定。对分离菌株进行16S rRNA基因扩增和序列比对,进行细菌种属鉴定和同源性分析,采用微量肉汤稀释法进行细菌耐药性检测。结果当地近期无相同病例发生,患儿无类似病例接触史,发病前已完成无细胞百白破联合疫苗基础免疫。患儿咽部病灶及其母亲咽部的拭子培养物中均分离到拥挤棒状杆菌。该菌株生化反应不活泼,对青霉素、红霉素、克林霉素、头孢西丁、氯霉素等存在不同程度的耐药。结论本调查实验室确诊了由拥挤棒状杆菌感染引起的白喉样病例,在国内外均为首次报道,细菌的致病性及多重耐药现象应引起重视。
[Abstract]:Objective to investigate the epidemiology and etiology of a case of diphtheria caused by Corynebacterium crowd.Methods Epidemiological investigation of diphtheria like cases and their close contacts was carried out in order to provide basis for clinical differential diagnosis and treatment. The samples of pharyngeal swabs were collected for bacterial isolation, culture and identification. The 16s rRNA gene was amplified and sequenced, and the species identification and homology analysis were carried out. Microbroth dilution method was used to detect bacterial resistance. Results there were no similar cases in the local area and no contact history of similar cases in the children. Before the onset of the disease, we have completed the basic immunization of acellular tetanus tetanus vaccine. Corynebacterium crowding was isolated from the swabs of the pharynx and the mother's pharynx. The strain was not active in biochemical reaction and reacted to penicillin, erythromycin and clindamycin. Conclusion this investigation laboratory confirmed diphtheria cases caused by Corynebacterium crowds. this is the first reported case both at home and abroad. Attention should be paid to the pathogenicity and multidrug resistance of bacteria.
【作者单位】：山东省疾病预防控制中心山东省传染病预防控制重点实验室;济南市传染病医院;中国疾病预防控制中心免疫规划中心;
【基金】：泰山学者工程专项经费资助
【分类号】：R516;R181.3

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