不同洗脱液对环氧乙烷生物指示剂活菌洗脱效果及抗力测定影响

发布时间：2018-03-19 23:24

本文选题：洗脱液　切入点：生物指示剂　出处：《中国消毒学杂志》2014年03期 　论文类型：期刊论文

【摘要】：目的研究不同洗脱液对环氧乙烷生物指示剂的菌量及抗力测定结果的影响。方法通过细菌定量检测方法,比较不同洗脱液对生物指示剂上活菌洗脱效果及其对生物指示剂抗力的影响。结果 6种洗脱液与5种稀释液对同一批次的生物指示剂载体上细菌洗脱后,细菌回收效果最好的是用5 000 mg/L吐温-80的营养肉汤洗脱后,用浓度为5 000 mg/L吐温-80的PBS,回收菌数为7.03×106cfu/片。回收效果最差的是无菌生理盐水,平均回收菌数为3.78×106cfu/片。回收菌数数值越大D值越小,ST值相应变大和KT值变小。结论洗脱效果最好的洗脱液和相应稀释液是吐温-80营养肉汤与吐温-80 PBS,生物指示剂回收菌数越高,其D值更接近真值。
[Abstract]:Objective to study the effect of different elution solution on bacterial quantity and resistance of ethylene oxide biological indicator. The effects of different eluents on the elution of living bacteria on biological indicators and their effects on the resistance of biological indicators were compared. Results six eluents and five diluents were used to elucidate bacteria on the same batch of biological indicator carriers. The best recovery effect of bacteria was after eluting with 5 000 mg/L Tween-80 nutrient broth and using 5 000 mg/L Tween-80 PBS.The number of bacteria recovered was 7.03 脳 106 cfu.The worst recovery effect was aseptic physiological saline. The average number of bacteria recovered was 3.78 脳 106 cfu.The higher the number of recovered bacteria, the smaller the St value and the smaller KT value. Conclusion the best eluent and dilution solution are Tween-80 nutrient broth and Tween-80 PBS, and the higher the number of bioindicator recovery bacteria is, the higher the recovery bacteria are. The D value is closer to the true value.
【作者单位】：江苏省疾病预防控制中心;江苏省人民医院;
【基金】：卫生部国家标准(2008-07-01) 江苏省“十二·五”科教兴卫工程(ZX201109):“突发公共卫生事件”应急处置创新平台
【分类号】：R187.8

【相似文献】