丙型肝炎病毒感染者F基因区变异临床意义的初步研究

发布时间：2018-03-22 00:28

  本文选题：肝炎　切入点：丙型　出处：《中华疾病控制杂志》2014年04期 　论文类型：期刊论文

【摘要】：目的探讨丙型肝炎病毒(hepatitis C virus,HCV)F基因区变异的临床意义。方法以78例未治疗慢性丙型肝炎患者为研究对象,采用单链构象多态性分析对HCV-F基因区进行准种分析,实时荧光定量聚合酶链反应对HCV RNA进行定量检测,利用酶联免疫吸附试验分别对患者血清中的抗F蛋白抗体、Th1(IL-12,IFN-γ)和Th2(IL-5,IL-10)细胞因子水平进行检测分析。结果 78例慢性丙型肝炎患者中有46例为抗HCV-F抗体阳性,阳性率为59%。HCV1b亚型患者F基因区准种数目高于非HCV1b亚型(P=0.002)。F基因区SSCP条带数与HCV RNA载量及ALT/AST水平呈正相关(均有P0.05)。另外,与F基因准种数量≤2的患者相比,F基因准种数量2的患者血清中,Th1细胞因子IL-12、IFN-γ水平降低(P=0.023,P=0.010),Th2细胞因子IL-5、IL-10水平显著升高(P=0.024,P=0.012)。结论 HCV-F基因区准种多样性与病毒复制、肝损伤和机体Th2优势应答有关,具有一定的临床意义。
[Abstract]:Objective to investigate the clinical significance of HCV-F gene mutation in hepatitis C virus hepatitis C. methods 78 patients with untreated chronic hepatitis C were studied by single strand conformation polymorphism (SSCP). Real-time fluorescence quantitative polymerase chain reaction was used for quantitative detection of HCV RNA. Elisa was used to detect and analyze the levels of cytokines of anti-F protein antibody Th _ 1T _ (12) IFN- 纬) and Th _ (2) IL-5 ~ (5) IL-10) in serum of patients with chronic hepatitis C. results 46 of 78 patients with chronic hepatitis C were positive for anti HCV-F antibody. The positive rate was that the number of F gene quasispecies in 59%.HCV1b subtype was higher than that in non HCV1b subtype P0. 002. F gene SSCP bands were positively correlated with HCV RNA load and ALT/AST level (P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P < 0. 05). Compared with the patients with F gene quasispecies number 鈮，

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