携带qacEΔ1基因的铜绿假单胞菌对消毒剂抗力的观察

发布时间：2018-03-26 22:04

本文选题：耐消毒剂基因　切入点：铜绿假单胞菌　出处：《中国消毒学杂志》2014年05期

【摘要】：目的研究广东地区医院临床分离铜绿假单胞菌携带qacEΔ1基因的情况及qacEΔ1携带菌株对消毒剂抗力。方法采用定量杀菌试验和基因扩增法,对广东省部分医院临床分离铜绿假单胞菌进行实验室研究。结果从30株临床分离的铜绿假单胞菌中检出26株携带qacEΔ1基因,阳性率为86.67%。经有效碘浓度为200 mg/L的碘伏消毒液作用7 min,qacEΔ1基因阳性菌株存活率为7.7%;qacEΔ1基因阴性菌株存活率为0.0%。经浓度为300 mg/L苯扎氯铵作用7 min,qacEΔ1阳性菌株存活率为11.5%;qacEΔ1基因阴性菌株存活率为6.7%。经500 mg/L有效氯消毒液作用10 min,qacEΔ1阳性菌株存活率为19.2%;qacEΔ1基因阴性菌株存活率为10.0%。结论广东地区医院临床分离的铜绿假单胞菌耐消毒剂基因携带率较高,qacEΔ1基因阳性的铜绿假单胞菌对所试验的3种消毒剂的抗力稍高于qacEΔ1基因阴性的菌株。
[Abstract]:Objective to study the clinical isolates of Pseudomonas aeruginosa carrying qacE 螖 1 gene and the resistance of qacE 螖 1 to disinfectant. Methods quantitative germicidal test and gene amplification were used. A laboratory study on clinical isolates of Pseudomonas aeruginosa in some hospitals of Guangdong Province was carried out. Results 26 strains of Pseudomonas aeruginosa were found to carry qacE 螖 1 gene from 30 clinical isolates of Pseudomonas aeruginosa. The positive rate was 86.67. The survival rate of the positive strains of QAC E 螖 1 gene was 7 minutes after exposure to iodophor disinfectant solution with effective iodine concentration of 200 mg/L. The survival rate of the negative strain was 0.00.The survival rate of the positive strain was 11.55 QacE 螖 1 positive strain treated with 300 mg/L benzalkonium chloride for 7 minutes. The survival rate of negative strains was 6.7. The survival rate of positive strains was 19.2qacE 螖 1 when exposed to 500 mg/L available chlorine disinfectant solution for 10 minutes. Conclusion the disinfectant resistance gene of Pseudomonas aeruginosa isolated from Guangdong hospital is carried by the disinfectant gene of Pseudomonas aeruginosa. The resistance of Pseudomonas aeruginosa with positive QAC E 螖 1 gene to the three disinfectants tested was slightly higher than that of qacE 螖 1 negative strains.
【作者单位】：广州出入境检验检疫局;汕头市中心医院;汕头大学附属第一医院;广州市胸科医院;广东药学院附属第一医院;深圳市儿童医院;
【基金】：广东省自然科学基金资助项目(0612296)
【分类号】：R187

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