安阳市2011-2012年流感病毒核酸检测能力考核结果分析

发布时间：2018-03-30 15:23

本文选题：流感病毒　切入点：RT-PCR　出处：《现代预防医学》2014年02期

【摘要】：目的通过分析安阳市网络流感实验室2011-2012年参加的国家和省级流感质控考核工作的基本情况和考核结果,比较RT-PCR、单通道实时荧光PCR和双通道实时荧光PCR 3种检验方法在流感检测中的优缺点,以建立和完善应对流感PCR质控考核工作的实验策略。方法分别使用RT-PCR、单通道实时荧光PCR和双通道实时荧光PCR 3种方法对2011-2012年国家流感中心及河南省疾控中心组织的3次流感质控考核盲样进行鉴定和分型。结果 2011年河南省考核,单通道实时荧光PCR方法可一次性对全部盲样正确分型,而RT-PCR方法首次实验有2份盲样不能确定HA亚型。2011年和2012年国家考核,单通道和双通道实时荧光PCR各有1份样品不能确认亚型,其他均成功分型。对比发现,单通道和双通道实时荧光PCR方法检测灵敏度无显著差异。结论实时荧光PCR,特别是双通道实时荧光PCR较普通RT-PCR优势显著,是未来检测工作的发展方向。但因商品化试剂质量不稳定,在实际工作中应尽量使用2种以上不同厂家的产品以便互相印证,确保结果无误。
[Abstract]:Objective to analyze the basic situation and assessment results of national and provincial influenza quality control assessment in Anyang Network Influenza Laboratory from 2011-2012. The advantages and disadvantages of RT-PCR, single-channel real-time fluorescent PCR and dual-channel real-time fluorescent PCR in influenza detection were compared. In order to establish and perfect the experimental strategy for quality control and assessment of influenza PCR, RT-PCR, single-channel real-time fluorescent PCR and dual-channel real-time fluorescent PCR were used to organize the National Influenza Center and the Center for Disease Control and Prevention of Henan Province from 2011 to 2012. Results in 2011, Henan Province was assessed. The single channel real-time fluorescence PCR method can correctly classify all the blind samples at one time, while two blind samples from the first experiment of the RT-PCR method can not determine the HA subtype. One sample of single channel and two channel real-time fluorescence PCR could not confirm the subtype, the others were classified successfully. There was no significant difference in sensitivity between single channel and dual channel real-time fluorescent PCR. Conclusion Real-time fluorescence PCR, especially dual-channel real-time fluorescent PCR, is superior to conventional RT-PCR. But because the quality of commercial reagents is unstable, we should use more than two kinds of products from different manufacturers in order to verify each other and ensure the results are correct.
【作者单位】：河南省安阳市疾病预防控制中心;
【分类号】：R181.24

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