南昌国境口岸中华按蚊氟氯氰菊酯抗药性及其机制研究

发布时间：2018-04-04 15:17

本文选题：中华按蚊　切入点：氟氯氰菊酯　出处：《中国血吸虫病防治杂志》2017年02期

【摘要】：目的了解南昌昌北国际机场中华按蚊的氟氯氰菊酯抗药性,初步探索其产生机制。方法应用WHO成蚊生测法检测当地中华按蚊抗药性水平,将生测过程中即将死亡蚊虫归为敏感蚊,存活蚊虫归为抗性蚊,检测并比较两组蚊虫P450单加氧酶、谷胱甘肽巯基转移酶(GST)活性;同时记录每只敏感蚊生测死亡时间,对生测死亡时间与P450单加氧酶、GST活性进行相关分析。结果当地中华按蚊生测死亡率为59.5%;抗性蚊、敏感蚊、实验室敏感蚊的P450单加氧酶活性差异具有统计学意义(F=151.89,P0.01),其中抗性蚊敏感蚊实验室敏感蚊;但三者GST活性差异无统计学意义(F=0.72,P0.05);蚊虫生测死亡时间与P450单加氧酶活性呈正相关性,相关系数为0.88,回归方程为y=79.479+1.512x;生测死亡时间与GST活性不具有相关性。结论南昌昌北国际机场中华按蚊对氟氯氰菊酯已产生抗药性,P450单加氧酶活性增强可能是其抗药性产生的机制之一。
[Abstract]:Objective to investigate the resistance of Anopheles sinensis to cypermethrin in Nanchang North International Airport and to explore its mechanism.Methods the resistance of Anopheles sinensis (Anopheles sinensis) was detected by WHO bioassay. The dead mosquitoes were classified as sensitive mosquitoes and the surviving mosquitoes were classified as resistant mosquitoes. The P450 monooxygenase was detected and compared between the two groups.The activity of glutathione mercaptotransferase (GST) and the biological death time of each sensitive mosquito were recorded and the correlation analysis was carried out between the GST activity of P450 monooxygenase and that of P450 monooxygenase.Results the mortality rate of Anopheles sinensis was 59.5. The difference of P450 monooxygenase activity among resistant mosquitoes, sensitive mosquitoes and laboratory sensitive mosquitoes was statistically significant.However, there was no significant difference in GST activity among the three groups, and there was a positive correlation between the death time of mosquitoes and P450 monooxygenase activity, the correlation coefficient was 0.88, and the regression equation was YJ79.479 1.512x.There was no correlation between the bioassay death time and the activity of GST.Conclusion the increase of P450 monooxygenase activity of Anopheles sinensis in Nanchang North International Airport may be one of the mechanisms of its resistance to cypermethrin.
【作者单位】：江西出入境检验检疫局保健中心;中国疾病预防控制中心寄生虫病预防控制所;
【基金】：质检总局科技计划应用技术研究类项目(2015IK163)
【分类号】：R184.31

【相似文献】