甘肃省2013～2016年手足口病病原学监测分析

发布时间：2018-04-09 13:04

本文选题：手足口病(HFMD)　切入点：病原学监测　出处：《病毒学报》2017年04期

【摘要】：本研究对甘肃省2013~2016年手足口病病原监测结果进行分析,以了解甘肃省这几年病原谱的构成,主要病原的流行特点,为进一步做好手足口病防控工作提供依据。2013~2016年,甘肃省监测网络实验室对7 171份标本进行了核酸检测,对其中3373份阳性标本开展了病毒分离培养,对得到的阳性分离株进行全长VP1区核苷酸序列测定及其分子特征分析。检测到核酸阳性标本4 314份,EV71为1 153份,占病原构成的27.73%,CVA16为1 452份,占33.66%,其他肠道病毒为1 709份,占39.62%。重症病例124例,阳性97例,其中EV71为23例,占病原构成的23.71%,CVA16为9例,占9.28%,其他肠道病毒为64例,占65.98%,EV71+CA16混合型为1例,占1.03%;死亡病例2例,均为EV71。2013年以其他肠道病毒为主要病原,占36.76%(347/944);2014年以CVA16为主,占52.83%(625/1 183);2015年和2016年均以其他肠道病毒为主,分别占57.04%(531/931)和48.25%(606/1 256)。分离到的病毒株除了EV71和CVA16,其他肠道病毒以CVA6、CVA10和CVA4为主。2013~2016年中,不同年份流行的病原不尽相同,不同地区流行的主要病原也存在地区差异。对流行的几种主要病原的VP1区分析结果表明:C4a基因亚型EV71在甘肃省仍然持续流行;CVA16的流行则是B1b基因亚型占据优势,B1a基因亚型逐渐消失;甘肃省流行的CVA6和CVA10与国内流行的毒株亲缘关系相近,且存在不同的传播链。
[Abstract]:This study analyzed the results of hand, foot and mouth disease surveillance in Gansu Province from 2013 to 2016, in order to understand the composition of pathogen spectrum and the epidemic characteristics of the main pathogens in Gansu Province in recent years, and to provide the basis for the prevention and control of hand, foot and mouth disease in Gansu Province from 2013 to 2016.The nucleic acid was detected in 7 171 samples from Gansu Provincial Surveillance Network Laboratory, of which 3373 positive samples were isolated and cultured. The nucleotide sequence of the full-length VP1 region and the molecular characteristics of the positive isolates were analyzed.There were 1 153 nucleic acid positive specimens (1 153), 14 452 (33. 6666) were CVA16 and 1 709 (39. 62%) other enterovirus.Among 124 severe cases, 97 were positive, including 23 cases of EV71, 9 cases (9.28%) of CVA16, 64 cases of other enterovirus, 1 case of mixed type of CA16 of 65.98% EV71 (1.033%), 2 cases of death.The analysis of the VP1 region of several major pathogens showed that the prevalence of EV71 was still prevalent in Gansu Province, but the dominant subtype of B1b gene was gradually disappeared.The relationship between CVA6 and CVA10 in Gansu Province was similar to that in China, and there were different transmission chains.
【作者单位】：甘肃省疾病预防控制中心;中国疾病预防控制中心病毒病预防控制所卫生部医学病毒和病毒病重点实验室;
【基金】：国家“十二五”艾滋病和病毒性肝炎等重大传染病防治科技重大专项(项目号:2012ZX10004-208),题目:甘肃及周边省区传染病病原谱流行规律研究甘肃省卫生行业科研管理项目(项目号:GWGL2014-75),题目:甘肃省手足口病病原变异变迁研究~~
【分类号】：R181.8;R725.1

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