HBV感染标志的血清流行病学研究及重组酵母乙肝疫苗成人近期免疫效果研究

发布时间：2018-04-14 05:11

本文选题：乙肝感染标志 + 血清流行病学　；参考：《河北医科大学》2005年硕士论文

【摘要】：目的:了解山东济阳县15~40 岁人群HBV 感染标志物的分布情况;观察国产10μg/ml 酵母乙肝疫苗不同免疫程式接种成人的免疫效果;分析单纯核心抗体阳性血清HBV DNA 阳性率。方法:2003 年11 月,在山东省济阳县整群随机抽取某镇20 个村,对15~40 岁人群采集静脉血。用固相放射免疫(SPRIA)法检测HBsAg、抗-HBs 和抗-HBc 等HBV 感染指标,用酶联免疫(EIA)法检测HBeAg 和抗-HBe 指标,分析当地的HBV 流行情况。将筛检出的易感者随机整群分为两组,分别按0-1-6 个月程序和0-6 个月程序接种10μg/ml 酵母乙肝疫苗。全程免疫后1 个月,SPRIA 法定量检测抗体水平。对收集的资料用Access 建立数据库,采用SAS8.0 进行统计学分析。对单独核心抗体阳性者用PCR 法检测HBV DNA。实验中以加热法提取血清中的HBV DNA,作为模板,扩增出S区序列,电泳鉴定。结果:横断面调查显示,15~40 岁人群HBsAg 流行率、抗-HBs 流行率、抗-HBc 流行率和HBV 总感染率分别为6.52%、24.77%、30.99%和43.63%。HBsAg 流行率随年龄的增长而升高(P0.05)。HBeAg 的流行率随年龄的增长而逐渐
[Abstract]:Objective : To investigate the distribution of HBV infection markers in people aged 15 to 40 years in Jiyang county , Shandong province , and observe the immune effect of 10 渭g / ml yeast hepatitis B vaccine in adults ; and analyze the positive rate of HBV DNA in the positive serum of pure core antibody .

Methods : In November 2003 , 20 villages in a town were randomly selected in Jiyang County , Shandong Province . Venous blood was collected from 15 - 40 years old . HBV infection indexes such as HBsAg , anti - HBs and anti - HBV were detected by solid - phase radioimmunoassay ( SPRIA ) . HBeAg and anti - HBe indexes were detected by EIA . The local HBV prevalence was analyzed .

The susceptible subjects were randomly divided into two groups : 0 - 1 - 6 - month procedure and 0 - 6 - month routine inoculation with 10 渭g / ml yeast hepatitis B vaccine . After the whole course of immunization , the antibody level was detected by SPRIA .

The collected data was established in Access database , and SAS 8.0 was used for statistical analysis .

HBV DNA was detected by polymerase chain reaction ( PCR ) method . HBV DNA was extracted from serum by heating method . The S - region sequence was amplified by electrophoresis and identified by electrophoresis .

Results : The prevalence rate of HBsAg , the prevalence of anti - HBs , the prevalence and the total HBV infection rate were 6.52 % , 24.77 % , 30.99 % and 43.63 % , respectively . The prevalence of HBsAg increased with age ( P0.05 ) . The prevalence of HBeAg increased with age .

【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R181.3;R186

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