婴儿BCG接种后免疫状态及接种效果与DC-SIGN基因多态性的关系

发布时间：2018-04-19 22:26

本文选题：卡介苗 + 细胞免疫　；参考：《暨南大学》2006年硕士论文

【摘要】：目的分析汉族婴儿接种卡介苗(BCG)后细胞免疫功能变化，探讨DC-SIGN编码基因第4外显子多拷贝重复序列区(简称DC-SIGN重复序列区基因)多态性与BCG接种效果之间的关系，为结核感染的防治提供新途径。方法收集150例BCG接种后PPD阳性汉族婴儿(接种成功组)和50例BCG接种后PPD阴性汉族婴儿(接种失败组)的外周静脉血，ELISA法测定血清细胞因子IFN-γ和IL-10的含量，评价细胞免疫功能；应用分子生物学技术(PCR扩增、琼脂糖凝胶电泳)分析DC-SIGN重复序列区基因多态性，探讨BCG接种效果与DC-SIGN码基因多态性的关系。结果 ①接种成功组和接种失败组血清细胞因子IFN-γ含量分别为49.78±12.20pg／ml和38.08±7.68pg／ml，差异具有统计学意义(P=0.001)；血清IL-10含量分别为3.60±2.00pg／ml和5.71±2.71pg／m，差异具有统计学意义(P=0.008)。②200例BCG接种汉族婴儿(包括接种成功和失败组)中，存在6和7型2种DC-SIGN重复序列区基因型，分别组成7／7野生型纯合子(197例，占98.5％)或6／7变异型杂合子(3例，，占1.5％)。③150例接种成功组婴儿的DC-SIGN重复序列区基因均为7／7纯合子(100％)；50例接种失败组的DC-SIGN重复序列区基因中，47例为7／7纯合子(94.0％)，3例为6／7杂合子(6.0％)。两组比较，差异有统计学意义(Fisher's精确概率法，P=0.015) 。结论 ①BCG接种成功(PPD阳性)婴儿的细胞免疫功能增强。②汉族婴儿DC-SIGN重复序列区基因以7／7野生型纯合子为主；BCG接种失败汉族婴儿存在6／7变异型杂合子。③DC-SIGN重复序列区基因变异可能是导致BCG接种失败的原因之一。
[Abstract]:Objective to analyze the changes of cellular immune function in infants of Han nationality after BCG inoculation, and to explore the relationship between the polymorphism of DC-SIGN repeat region in exon 4 of DC-SIGN coding gene and the effect of BCG inoculation. To provide a new way for the prevention and treatment of tuberculosis infection. Methods Serum levels of cytokines IFN- 纬 and IL-10 were determined by Elisa in 150 PPD positive Han infants (successful group) and 50 PPD negative Han infants (failed group) after BCG inoculation. To evaluate the cellular immune function, to analyze the polymorphism of DC-SIGN repeat region by molecular biological technique and agarose gel electrophoresis, and to explore the relationship between the effect of BCG inoculation and the polymorphism of DC-SIGN code gene. Results 1the levels of serum cytokine IFN- 纬 in successful inoculation group and failed inoculation group were 49.78 卤12.20pg/ml and 38.08 卤7.68 PG / ml, respectively, the difference was statistically significant (P < 0.01), and the serum IL-10 content was 3.60 卤2.00pg/ml and 5.71 卤2.71pggm-1, respectively. The difference was statistically significant in BCG inoculated Han nationality infants. In children (including successful and failed vaccination groups), There were two genotypes of 6 and 7 DC-SIGN repeats, which consisted of 7 / 7 wild type homozygotes in 197 cases (98.5%) or 6 / 7 variant heterozygotes in 3 cases, respectively. The DC-SIGN repeat region genes in 1.5% and 3150 cases of successful inoculation group were 7 / 7 homozygote and 50 cases of failed inoculation group. 47 cases were 7 / 7 homozygous 7% homozygous 3 cases were 6 / 7 heterozygote 6.0g. The difference between the two groups was statistically significant (P 0.015). Conclusion the cellular immune function of infants with successful 1BCG inoculation is enhanced. 2 the gene of DC-SIGN repeat region in Han infants is mainly 7 / 7 wild type homozygote. There is 6 / 7 heterozygote. 3DC-SIGN heavy in Han infants. The gene variation of complex sequence region may be one of the reasons for the failure of BCG inoculation.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R186

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