2013年杭州地区手足口病流行现状及检测的方法学分析

发布时间：2018-05-01 13:20

本文选题：手足口病 + 柯萨奇病毒　；参考：《中国卫生检验杂志》2014年11期

【摘要】：目的研究2013年杭州地区儿童手足口病流行特征,比较2种实验室检测方法的检出率。方法搜集杭州流行期间临床疑似手足口病的患儿283名,同时采集咽拭子或肛拭子标本和急性期血液样本,荧光定量PCR测定咽拭子或肛拭子标本中的肠道病毒;ELISA测定急性期血清中EV71型IgM抗体和CA16型IgM抗体。整理2011年-2013年本院HFMD的数据,统计不同类型肠道病毒的分布比例和流行趋势。结果近3年EV71和CA16有下降趋势,其他EV比例2013年明显上升。2013年发病年龄在0岁~3岁的患儿占86.22%,男女比例为1.44∶1。PCR的总阳性率为84.10%,EV71、CA16和其他EV分别占27.21%(77/283),4.59%(13/283)和52.30%(148/283);ELISA检测的总阳性率为32.51%(92/283),其中EV71和CA16-IgM分别占29.33%(83/283)和3.18%(9/283)。两种方法结果的配对χ2检验P0.05。结论 2013年春夏季杭州地区手足口病流行特点以其他EV为主,以发热等轻症为主;引起重症HFMD以EV71型为主。PCR与ELISA对EV71和CA16的检出率差异无统计学意义,ELISA适合基层推广。
[Abstract]:Objective to study the epidemic characteristics of hand, foot and mouth disease (HFMD) in children in Hangzhou in 2013 and to compare the detection rate of two laboratory methods. Methods 283 children with suspected HFMD during epidemic period in Hangzhou were collected, and throat swabs or anal swabs and blood samples in acute phase were collected. Serum EV71 type IgM antibody and CA16 type IgM antibody were detected by fluorescence quantitative PCR in throat swabs or anal swabs by Elisa. The data of HFMD from 2011 to 2013 were collected and the distribution and epidemic trend of different types of enterovirus were analyzed. Results EV71 and CA16 showed a downward trend in the last three years. The proportion of other EV increased significantly in 2013. In 2013, 86.22 cases were aged 0 to 3 years old, and the total positive rate of EV71CA16 and other EV levels was 27.2177 / 283C / 283Q / 13283 and 52.30148283P / 92833, respectively. The total positive rates of EV71 and CA16-IgM were 29.3383 / 283and 3.18 / 9283respectively, respectively. The positive rate of EV71CA16 and other EV was 27.21 / 283and 52.30148283P / 928 / 283respectively, and the positive rates of EV71 and CA16-IgM were 29.3383 / 283and 3.18 / 9283respectively. The paired 蠂 2 test of the results of the two methods (P 0.05). Conclusion the epidemic characteristics of HFMD in Hangzhou in spring and summer 2013 are mainly other EV and mild fever, and the detection rate of EV71 and CA16 is not significantly different between ELISA and EV71.
【作者单位】：杭州市儿童医院检验科;杭州市儿童医院感染科;
【基金】：浙江省自然科学基金(LY13H040007) 浙江省医药卫生科技计划(2012KYB159)
【分类号】：R181.3

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