三氯羟基二苯醚抗菌涂膜对导尿管生物膜抑制作用的实验观察

发布时间：2018-05-02 13:12

本文选题：三氯羟基二苯醚 + 生物膜　；参考：《中国消毒学杂志》2017年02期

【摘要】：目的研究三氯羟基二苯醚(三氯生)抗菌涂膜抑制导尿管细菌生物膜的效果。方法采用扫描电镜法和抑菌环试验法,对导尿管细菌生物膜的形成和三氯生抗菌涂膜抑制生物膜的效果进行观察。采用浸泡法在导尿管表面形成抗菌涂层。通过构建细菌生物膜模型,采用形态显微表征、静态接触角测试、细菌生物膜生长曲线测定、抑菌效果检测等实验方法,了解导尿管表面细菌生物膜的生成状况,评价抗菌涂层对导尿管表面生物膜形成的干预效果。结果以浓度500 mg/L三氯生制备的抗菌涂膜导尿管对金黄色葡萄球菌和粪肠球菌的抑菌环直径分别为51 mm和43 mm;将抗菌导尿管浸泡在金黄色葡萄球菌和粪肠球菌的营养肉汤中从第2 d开始逐渐显示出明显抑菌生物膜的形成,抑菌作用可持续至第8 d。扫描电镜下显示,在菌悬液内浸泡8 d的抗菌导尿管表面无生物膜形成,而对照组导尿管表面形成了明显的生物膜。结论三氯生抗菌涂膜能够持续有效地抑制金黄色葡萄球菌和粪肠球菌在导尿管表面形成生物膜。
[Abstract]:Objective to study the inhibitory effect of trichloro-hydroxydiphenyl ether (trichloro-hydroxydiphenyl ether) on bacterial biofilm in urethral catheter. Methods the formation of bacterial biofilm in urethral catheter and the inhibitory effect of triclosan coating on biofilm were observed by scanning electron microscopy and bacteriostatic ring test. An antimicrobial coating was formed on the surface of the catheter by immersion. The formation of bacterial biofilm on the surface of urethral catheter was studied by constructing bacterial biofilm model, using morphological microscopic characterization, static contact angle test, bacterial biofilm growth curve measurement, bacteriostasis test and so on. To evaluate the effect of antibacterial coating on the formation of biofilm on the surface of urethral catheter. Results the antimicrobial film catheter prepared with 500 mg/L trichlorogenic was used to inhibit Staphylococcus aureus and Enterococcus faecalis respectively, and the antibacterial catheter was immersed in the camp of Staphylococcus aureus and Enterococcus faecalis. From the second day, the formation of bacteriostatic biofilm was gradually shown in broth. The bacteriostasis lasted until the 8th day. SEM showed that no biofilm was formed on the surface of antibacterial urethral catheter after 8 days immersion in bacteria suspension, but obvious biofilm was formed on the surface of urethral catheter in control group. Conclusion triclosan antibacterial film can effectively inhibit the formation of biofilm on the surface of urethral catheter by staphylococcus aureus and Enterococcus faecalis.
【作者单位】：湖北省疾病预防控制中心;
【基金】：中华预防医学会消毒领域科研创新专项(2013ZY003)
【分类号】：R187

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