狂犬病疫苗与蟾皮提取液联合应用预防狂犬病效果的研究

发布时间：2018-05-08 17:49

本文选题：狂犬病疫苗 + 纯化　；参考：《吉林大学》2007年硕士论文

【摘要】： 蟾皮作为传统中药的重要药材,在咳喘、肝硬化腹水、湿疹、肿瘤、疳积、咽喉肿痛、痈肿等诸多病症的治疗中起举足轻重的作用。在这些疾病的治疗中蟾皮的免疫学作用日益体现出来,但由于现有蟾皮制剂有一定得副作用,不能满足用于免疫治疗制剂对安全性的要求,未在免疫学领域广泛应用。本试验对蟾皮提取液进行了初步纯化,去除刺激性较大的成分以提高其安全性。狂犬病作为目前我国传染病疫情中造成死亡人数最多的传染病,目前还没有找到有效的治疗方法。暴露后以狂犬病疫苗免疫是主要的预防手段,能否产生高效价的中和性抗体和良好的细胞免疫对狂犬病的防治起到至关重要的作用。本文进一步纯化蟾皮提取液,并与狂犬病疫苗在动物中联合应用,观察其细胞免疫效果,指标有脾淋巴细胞转化率,CD4+、CD4+/CD8+,巨嗜细胞吞噬率和吞噬指数。同时观察其体液免疫效果,测定不同时间的中和抗体效价。经统计学分析狂犬病疫苗与蟾皮提取物联合应用具有明显增强机体细胞免疫的功能,能够快速产生抗病毒抗体,对主动的细胞免疫有很好的协同作用。
[Abstract]:As an important traditional Chinese medicine, Xenopus skin plays an important role in the treatment of cough and asthma, cirrhotic ascites, eczema, tumor, infantile, throat swelling, carbuncle and so on. The immunological effect of Xenopus skin is increasingly reflected in the treatment of these diseases, but the existing Xenopus skin preparations have some side effects, which can not meet the safety requirements of immunotherapy agents, and have not been widely used in immunological field. In order to improve the safety of Xenopus skin extract, the extract of Xenopus skin was preliminarily purified and the irritant components were removed. Rabies is one of the most common infectious diseases in China. Immunization with rabies vaccine after exposure is the main preventive method. It is very important to produce high titer neutralizing antibody and good cellular immunity for the prevention and treatment of rabies. In this paper, the extract of Xenopus skin was further purified and used in combination with rabies vaccine in animals to observe its cellular immune effect. The indexes included spleen lymphocyte transformation rate, CD4 / CD8, phagocytosis rate and phagocytic index of giant eosinophils. At the same time, the humoral immune effect was observed, and the neutralizing antibody titer at different time was determined. The combination of rabies vaccine and Xenopus skin extract can obviously enhance the function of cellular immunity and produce anti-virus antibody quickly. It has a good synergistic effect on active cellular immunity.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R186;R285.5

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