湖北省大流行期和流行后期新甲型H1N1流感病毒基因特性分析

发布时间：2018-05-10 05:43

本文选题：流感病毒A型 + HN亚型　；参考：《中华疾病控制杂志》2017年01期

【摘要】：目的了解湖北省大流行期和流行后期新甲型H1N1流感病毒在流行分布、基因进化、抗原表位和耐药位点等方面的差异情况。方法对2009-2014年湖北省大流行期和流行后期新甲型H1N1流感病毒血凝素和神经氨酸酶的系统进化树、进化速率和氨基酸突变位点进行对比分析。结果大流行期病毒核酸阳性率大于流行后期,但大流行期病毒血凝素和神经氨酸酶基因进化速率均小于流行后期,且神经氨酸酶进化速率在两个时期都大于血凝素。血凝素抗原表位突变位点K163Q、A186T、S185T和S203T在大流行期与流行后期病毒的分布数和位置存在不同偏嗜,流行期鲜有的H275Y耐药位点在流行后期的三株病毒中被发现。结论流行后期的新甲型H1N1流感病毒进化速率不断提高,抗原表位和神经氨酸酶抑制剂耐药位点不断积累,防控工作愈加严峻,需进一步加强该病毒基因特性和耐药分析的监测。
[Abstract]:Objective to investigate the distribution, gene evolution, epitope and drug resistance of new influenza A (H1N1) virus in Hubei Province. Methods the phylogenetic tree, evolutionary rate and amino acid mutation sites of new A H1N1 influenza virus hemagglutinin and neuraminidase from 2009 to 2014 in Hubei Province were compared and analyzed. Results the nucleic acid positive rate of the virus in the large epidemic period was higher than that in the late epidemic period, but the evolution rate of the virus hemagglutinin and neuraminidase genes in the large epidemic period was lower than that in the latter stage of the epidemic, and the evolution rate of neuraminidase was higher than that of hemagglutinin in both periods. Hemoagglutinin antigen epitope mutation sites K163QA186TnS185T and S203T were different in the distribution and location of H275Y, and the H275Y resistance sites were found in three strains of viruses in the later stage of epidemic. Conclusion the evolutionary rate of the new influenza A H1N1 virus in the late stage of epidemic is increasing, the antigenic epitopes and neuraminidase inhibitor resistance sites are accumulating, and the prevention and control work is becoming more and more severe. The monitoring of the gene characteristics and drug resistance analysis of the new influenza A virus should be further strengthened.
【作者单位】：湖北省疾病预防控制中心卫生检验检测研究所流感参比实验室;
【基金】：“十二五”国家科技重大专项(2012ZX10004-207)
【分类号】：R511.7;R181.3

【相似文献】