特定人群及动物巴尔通体感染状况及流行病学调查

发布时间：2018-05-18 19:03

本文选题：巴尔通体 + 分子鉴定　；参考：《中国疾病预防控制中心》2010年硕士论文

【摘要】： 目的：调查特定人群(职业性动物接触人群、犬伤或猫伤人群)及不同来源的动物(家养宠物、流浪动物、实验动物)巴尔通体感染状况及流行病学信息,获得不同宿主的巴尔通体分离株,了解并比较巴尔通体感染率及分布情况,探索巴尔通体感染的危险因素。方法：在山东省潍坊市和北京市采集193份人血样与478份动物血样,并收集其相关的流行病学信息。将采获的样本抗凝血接种于含5%去纤维羊血的脑心浸液培养基上,置于37度、含5%CO2培养箱中分离培养。挑选巴尔通体疑似菌落,应用聚合酶链反应技术(PCR)在属分类水平鉴定巴尔通体,选择gltA、16S、16S-23S rRNA ITS、ftsZ、ribC引物进行PCR扩增并测序,将所测核酸序列进行同源性比较及系统发育分析,确定巴尔通体种。提取血样DNA并进行gltA、tRNA引物PCR扩增、测序序列进行同源性分析。利用抗巴尔通体抗体IgG检测试剂盒检测样本血清中抗体水平。利用SPSS分析实验室结果与现场采集的流行病学信息之间关系。结果： 1、不同人群之间(貂狐饲养者、犬伤或猫伤人群、实验动物饲养人员)巴尔通体血清阳性率没有显著性差异,平均为33.7%。犬伤或猫伤人群巴尔通体血清阳性率有在不同地区显著性差异,城区犬伤或猫伤人群血清阳性率(56.4%)明显高于农村地区(11.9%),且仅有猫接触史的咬伤人群的血清阳性率(83.3%)显著高于仅有犬接触史的咬伤人群(25.8%)。不同类型动物接触史(家养宠物、职业性接触、流浪动物)、不同性别、不同单位、不同年龄、不同工龄的调查人群巴尔通体血清阳性率没有显著性差异。 2、本课题获得的22株猫分离株全部为汉赛巴尔通体。流浪猫带菌率(30.4%)明显高于家养宠物猫(4.8%),但两者血清阳性率没有显著性差异,平均为39.4%。寄生虫感染猫的带菌率(36.6%)要明显高于无寄生虫感染猫(5.9%)。幼猫带菌率(27.6%)明显高于性成熟猫(10.7%),不同年龄的猫带菌情况不同,以一岁以内的小猫带菌率最高(27.9%)。不同品种、来源、性别、体重的猫之间巴尔通体感染状况没有显著性差异。 3、间接免疫荧光法(IFA)在诊断猫现时感染时,灵敏度(77.3%)和特异度(66.7%)一般。IFA的阴性预测值较高(94.8%),既当IFA阴性时,有较大的把握该猫不带有菌血症。 4、本课题获得国内首株汉赛巴尔通体犬分离株,犬一般感染文森巴尔通体,获得汉赛巴尔通体犬分离株对巴尔通体的宿主研究提出方向。 5、本课题获得国内首株五日热巴尔通体分离株,这也是国际首次在猕猴体内发现自然感染的五日热巴尔通体。这证实国内存在战壕热病原体,提示巴尔通体的流行病学可能比预期的要复杂,对巴尔通体宿主特异性的进一步研究提出方向。
[Abstract]:Objective: to investigate the prevalence and epidemiological information of Bartonella infection in specific population (occupational animal contact population, canine injury or cat injury population) and animals from different sources (domestic pets, stray animals, laboratory animals). Baltonella isolates from different hosts were obtained, and the infection rate and distribution of Baltonella were compared and the risk factors of Bartonella infection were explored. Methods: 193 human and 478 animal blood samples were collected in Weifang and Beijing, Shandong Province. The collected anticoagulant blood was inoculated on the medium containing 5% defibrillated sheep blood and incubated in 37 degrees in a 5%CO2 incubator. The suspected colony of Bartonella was selected and identified at the taxonomic level by polymerase chain reaction (PCR). The primers of gltAmin16Sn16S-23S rRNA ITSftsZribC were selected for PCR amplification and sequencing. Homology and phylogenetic analysis of the nucleic acid sequences were carried out. To determine the species of Bartonella. DNA was extracted from blood samples and amplified by PCR with gltAgna tRNA primer, and the sequence was sequenced for homology analysis. The antibody level in serum was detected by anti-Bartonella antibody IgG assay kit. The relationship between the laboratory results and the epidemiological information collected in the field was analyzed by SPSS. Results: 1. There was no significant difference in the positive rate of Baltonella among different populations (mink breeders, dogs or cats, laboratory animal breeders), with an average of 33.7. The positive rates of Baltonella in dogs and cats were significantly different in different areas. The positive rate of serum in dogs or cats in urban areas was significantly higher than that in rural areas (11.9%), and the positive rate of 83.3% in people with only cat contact history was significantly higher than that in the group with only dog contact history (25.8%). There was no significant difference in the positive rate of Baltonella among different types of animals (domestic pets, occupational contact, stray animals, different sex, different units, different ages and different years of service). 2. The 22 cat isolates in this study were all Hansebartonella. The bacterial rate of stray cats (30.4%) was significantly higher than that of domestic pet cats (4.8%), but there was no significant difference in serum positive rate between the two groups (mean 39.4%). The bacterial rate of parasitic infected cats was significantly higher than that of non-parasitic infected cats (36.6%). The rate of carrying bacteria in kittens was significantly higher than that in sexually mature cats (10.7%). The rate of carrying bacteria in kittens of different ages was different, and the highest rate of carrying bacteria was 27.9% in kittens under one year of age. There was no significant difference in Bartonella infection among cats of different breeds, sources, gender, and body weight. (3) the sensitivity and specificity of indirect immunofluorescence assay (IFA) in the diagnosis of current infection in cats were 77.3) and 66.7). The negative predictive value of IFA was higher than that of normal. When IFA was negative, there was a greater certainty that the cat did not have bacteremia. 4. In this study, the first strain of Hanseybaltonella canis was obtained, and the canine strain was generally infected with Venson Bartonella, and the direction of research on the host of Bartonella was put forward. In this study, the first 5-day hot Baltonella isolate was obtained in China, which was the first time that natural infection was found in rhesus monkeys. This confirms the existence of trench fever pathogens in China, suggesting that the epidemiology of Bartonella may be more complicated than expected.
【学位授予单位】：中国疾病预防控制中心
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R181.3

【引证文献】