ATP生物荧光法检测染菌量的可行性研究

发布时间：2018-05-29 19:07

本文选题：ATP生物荧光法 + ATP细菌计数　；参考：《中国消毒学杂志》2014年09期

【摘要】：目的研究ATP生物荧光法监测物体表面染菌量的可行性。方法采用ATP生物荧光仪及其配套试剂检测方法,对医务人员手上污染菌量进行监测,同时与活菌计数培养法作平行比较。结果洗手前后、消毒前后检测的ATP总量、ATP细菌计数和菌落计数均有显著性差异,手消毒后的ATP总量高于消毒前,ATP细菌计数法与比浊仪标定的菌悬液浓度在同一数量级。结论 ATP生物荧光法可用于监测手等物体表面的污染程度,但ATP总量测定不适用于监测手消毒的效果。ATP细菌计数由于消除了体细胞ATP对检测结果的影响,可以用于手消毒效果的监测。
[Abstract]:Objective to study the feasibility of ATP biofluorescence method for monitoring bacterial contamination on the surface of objects. Methods ATP biofluorometer and its reagent were used to monitor the contamination of bacteria in the hands of medical workers, and the results were compared in parallel with the method of counting and culture of live bacteria. Results before and after hand washing, there were significant differences in total ATP bacterial count and colony count before and after disinfection. The total ATP content after hand disinfection was higher than that before disinfection by bacteria count method and turbidimeter at the same order of magnitude. Conclusion the method of ATP biofluorescence can be used to monitor the degree of contamination on the surface of hand and other objects, but the total amount of ATP is not suitable for monitoring the effect of hand disinfection. The count of ATP bacteria eliminates the influence of somatic ATP on the result of detection. It can be used to monitor the hand disinfection effect.
【作者单位】：河北医科大学第二医院;
【分类号】：R187

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