糖尿病小鼠接种流感病毒灭活疫苗和DNA疫苗的保护作用研究

发布时间：2018-06-04 09:51

本文选题：流感病毒 + 糖尿病　；参考：《湖南师范大学》2005年硕士论文

【摘要】：本论文由三部分组成:种系和性别因素在小鼠对链脲佐菌素(streptozotocin,STZ)诱发型糖尿病感受性中的影响;糖尿病小鼠接种流感灭活疫苗的免疫应答及其保护作用;糖尿病小鼠接种流感DNA疫苗的免疫应答及其保护作用。 (一) 种系和性别因素在小鼠对链脲佐菌素诱发型糖尿病感受性中的影响。链脲佐菌素可以诱导小鼠产生与人糖尿病相似的高血糖症状。用不同剂量的STZ对昆明小鼠、BALB/c小鼠腹腔注射,定期检测其血糖。结果发现昆明小鼠雌雄均能诱发出高血糖症状,但雄性对STZ更为敏感;在BALB/c中,雄性小鼠能成功建成Ⅰ型糖尿病模型,而雌性不能成模。这说明种系和性别因素在决定小鼠对STZ诱导产生糖尿病的敏感性方面有重要作用。 (二) 糖尿病小鼠接种流感灭活疫苗的免疫应答及其保护作用。利用糖尿病BALB/c小鼠模型,分别用不同剂量流感灭活疫苗免疫正常小鼠和糖尿病小鼠一次或两次,ELISA方法测血清的特异性IgG抗体量,用致死量流感病毒感染后,观察小鼠的体重变化和存活率。结果表明,糖尿病小鼠对流感灭活疫苗的抗体反应有一定程度的抑制现象;一次较高剂量或两次较低剂量疫苗免疫可以较好的保护糖尿病小鼠免受致死量流感病毒的侵害,而一次低剂量疫苗免疫就可提供健康小鼠完全的保护。 (三) 糖尿病小鼠接种流感DNA疫苗的免疫应答及其保护作用。分别用不同剂量流感HA或NA核酸疫苗免疫正常小鼠和糖尿病小鼠一次或两次,测定血清的特异性抗体量,用致死量流感病毒感染后,观察小鼠的体重变化和存活率。结果显示,糖尿病小鼠的抗体产生受到抑制。NA无论在健康小鼠或糖尿病小鼠中都有很好的保护作用,且低量免疫一次就已足够;而HA大剂量免疫两次也不能给予糖尿病小鼠足够的保护。
[Abstract]:This paper consists of three parts: the effect of strain and sex factors on the susceptibility of mice to streptozotocinia STZ, the immune response of diabetic mice to inactivated influenza vaccine and its protective effect; Immune response and Protective effect of Diabetic mice inoculated with Influenza DNA Vaccine. (1) the effect of strain and sex factors on the susceptibility of mice to streptozotocin induced diabetes mellitus. Streptozotocin can induce hyperglycemia in mice similar to human diabetes. Kunming mice were injected intraperitoneally with different doses of STZ and their blood glucose was measured regularly. The results showed that both male and female mice could induce hyperglycemia, but male mice were more sensitive to STZ. In BALB/c, male mice could successfully establish type 1 diabetic model, but female mice could not. These results suggest that germline and sex play an important role in determining the sensitivity of mice to STZ induced diabetes. (2) immune response and protective effect of inactivated influenza vaccine in diabetic mice. BALB/c mice were immunized with different doses of inactivated influenza vaccine once or twice to determine the specific IgG antibody in serum. The changes of body weight and survival rate of mice were observed. The results showed that the antibody response of diabetic mice to inactivated influenza vaccine was inhibited to a certain extent, and one dose or two doses of vaccine immunization could protect diabetic mice from the lethal dose of influenza virus. A single low-dose vaccination can provide complete protection in healthy mice. (3) immune response of diabetic mice to influenza DNA vaccine and its protective effect. Different doses of influenza HA or na nucleic acid vaccine were used to immunize normal mice and diabetic mice once or twice respectively. The specific antibody levels in serum were measured. The body weight and survival rate of mice were observed after influenza virus infection with lethal dose. The results showed that the antibody production in diabetic mice was inhibited. Na had a good protective effect in both healthy mice and diabetic mice. High doses of HA also failed to protect diabetic mice twice.
【学位授予单位】：湖南师范大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R186

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