江西省2009-2011年新型甲型H1N1流感病毒耐药性分析

发布时间：2018-07-07 08:52

本文选题：新型甲型HN流感病毒 + M　；参考：《现代预防医学》2014年09期

【摘要】：目的分析江西省2009-2011年甲型H1N1流感病毒M2以及NA基因的特点,掌握其耐药情况,为临床治疗和疾病控制提供参考。方法从江西省流感监测网中随机选择19株甲型H1N1流感病毒,经核酸提取和one-step RT-PCR扩增M以及NA基因片段,双向序列测定,采用DNAStar5.0和Mage4.0序列分析软件分析M2以及NA基因特征以及耐药性位点。结果 19株毒株M2基因发生了S31N的突变;仅A/JiangxiDonghu/SWL11171/2009(H1)株的NA蛋白发生了E119Q替换,其他毒株NA蛋白催化活性位点和辅助位点均未发生氨基酸替换。结论 19株毒株均对金刚烷胺类药物耐药,几乎都对流感病毒神经氨酸酶抑制剂药物敏感,但仍应加强对流感病毒的耐药性监测。
[Abstract]:Objective to analyze the characteristics of M2 and na genes of influenza A (H1N1) virus in Jiangxi Province from 2009 to 2011, and to understand the drug resistance of influenza A (H1N1) virus and to provide reference for clinical treatment and disease control. Methods 19 strains of influenza A (H1N1) virus were randomly selected from the influenza surveillance network in Jiangxi Province. M and na gene fragments were amplified by nucleic acid extraction and one-step RT-PCR. DNA Star5.0 and Mage 4.0 sequence analysis software were used to analyze the characteristics of M2 and na genes and drug resistance sites. Results there was S31N mutation in M2 gene of 19 strains, and E119Q substitution in na protein of A / JiangxiDonghu / SWL11171 / 2009 (H1) strain, and no amino acid substitution at catalytic active site and auxiliary site of na protein in other strains. Conclusion all the 19 strains are resistant to amantadine and almost all are sensitive to neuraminidase inhibitors of influenza virus, but the surveillance of influenza virus resistance should be strengthened.
【作者单位】：江西省疾病预防控制中心;
【基金】：江西省卫生厅科技计划(20112003)
【分类号】：R446.5;R181.3

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