多耐药铜绿假单胞菌对消毒剂抗性检测与抗性诱导的试验研究
发布时间：2018-07-23 10:39
【摘要】： [目的] 探讨医院感染频发的潜在因素之一细菌对消毒剂的抗性变化情况；对消毒剂抗性检测方法的试验过程进行规范,找出适合对消毒剂抗性进行长期监测的试验方法：通过完善的试验方法对多耐药铜绿假单胞菌的抗性进行检测；通过抗性诱导的方法观察多耐药铜绿假单胞菌对消毒剂抗性的变化情况。 [方法] 使用营养肉汤稀释法进行过氧乙酸、戊二醛、84消毒液、碘伏、新洁尔灭对多耐药铜绿假单胞菌最小抑菌浓度(MIC)的测定；使用MIC加中和剂法对多耐药铜绿假单胞菌进行最小杀菌浓度(MBC)的测定；通过递增浓度诱导法对戊二醛、碘伏、新洁尔灭进行抗性诱导试验。 [结果] 23株多耐药铜绿假单胞菌中发现了对戊二醛(1株)、新洁尔灭(7株)抗性大于标准菌株(比较MIC)的菌株,多耐药铜绿假单胞菌对不同消毒剂的抗性情况不同。未发现在常规使用的消毒剂浓度下不能杀灭的菌株。通过对戊二醛、碘伏、新洁尔灭抗性的诱导,均出现了抗性增强的现象。 [结论] 多耐药铜绿假单胞菌对常用消毒剂的抗性存在超过标准菌株的情况,由其引起的医院感染后果较严重,应引起足够的重视。研究中所选择的五种常用消毒剂在常规推荐使用浓度下均可杀灭试验菌株,提示正确使用消毒剂可以达到预防医院感染的目的。长时间接触亚致死剂量的消毒剂可以诱导对该种消毒剂的抗性增强,提示在日常消毒工作中应注意足量使用消毒剂,不能随意降低浓度长期使用。
[Abstract]:[objective] to investigate the change of bacterial resistance to disinfectants, one of the potential factors of frequent nosocomial infection, and to standardize the test process of the methods for detecting the resistance of disinfectants. To find out the suitable long-term monitoring method for the resistance of disinfectant: to detect the resistance of multidrug resistant Pseudomonas aeruginosa by perfect test method; The resistance of multidrug resistant Pseudomonas aeruginosa to disinfectant was observed by resistance induction. [methods] the minimal inhibitory concentration (MIC) of peracetic acid, glutaraldehyde (Glutaraldehyde) 84 disinfectant, iodophor and neugeramine against multidrug resistant Pseudomonas aeruginosa was determined by nutrient broth dilution method. The minimal bactericidal concentration (MBC) of multidrug resistant Pseudomonas aeruginosa was determined by MIC plus neutralization method, and the resistance induction test of glutaraldehyde, iodophor and neugericides was carried out by increasing concentration induction method. [results] among 23 strains of multidrug resistant Pseudomonas aeruginosa, glutaraldehyde (1 strain) and ninjelme (7 strains) were found to be more resistant than standard strains (MIC). The resistance of multidrug resistant Pseudomonas aeruginosa to different disinfectants was different. No strains were found that could not be killed at the concentration of conventional disinfectants. The resistance to glutaraldehyde, iodophor and nebulidene were all increased. [conclusion] the resistance of multidrug resistant Pseudomonas aeruginosa to common disinfectants is higher than that of standard strains, and the nosocomial infection caused by it is serious, which should be paid more attention to. The five commonly used disinfectants selected in the study could kill the tested strains at the recommended concentration, indicating that the correct use of disinfectants could prevent nosocomial infection. Long exposure to sublethal dose of disinfectant could induce the resistance of the disinfectant to the disinfectant. It suggested that sufficient amount of disinfectant should be paid attention to in the daily disinfection work, and the concentration of disinfectant should not be reduced at will for a long time.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R187
本文编号：2139129
[Abstract]:[objective] to investigate the change of bacterial resistance to disinfectants, one of the potential factors of frequent nosocomial infection, and to standardize the test process of the methods for detecting the resistance of disinfectants. To find out the suitable long-term monitoring method for the resistance of disinfectant: to detect the resistance of multidrug resistant Pseudomonas aeruginosa by perfect test method; The resistance of multidrug resistant Pseudomonas aeruginosa to disinfectant was observed by resistance induction. [methods] the minimal inhibitory concentration (MIC) of peracetic acid, glutaraldehyde (Glutaraldehyde) 84 disinfectant, iodophor and neugeramine against multidrug resistant Pseudomonas aeruginosa was determined by nutrient broth dilution method. The minimal bactericidal concentration (MBC) of multidrug resistant Pseudomonas aeruginosa was determined by MIC plus neutralization method, and the resistance induction test of glutaraldehyde, iodophor and neugericides was carried out by increasing concentration induction method. [results] among 23 strains of multidrug resistant Pseudomonas aeruginosa, glutaraldehyde (1 strain) and ninjelme (7 strains) were found to be more resistant than standard strains (MIC). The resistance of multidrug resistant Pseudomonas aeruginosa to different disinfectants was different. No strains were found that could not be killed at the concentration of conventional disinfectants. The resistance to glutaraldehyde, iodophor and nebulidene were all increased. [conclusion] the resistance of multidrug resistant Pseudomonas aeruginosa to common disinfectants is higher than that of standard strains, and the nosocomial infection caused by it is serious, which should be paid more attention to. The five commonly used disinfectants selected in the study could kill the tested strains at the recommended concentration, indicating that the correct use of disinfectants could prevent nosocomial infection. Long exposure to sublethal dose of disinfectant could induce the resistance of the disinfectant to the disinfectant. It suggested that sufficient amount of disinfectant should be paid attention to in the daily disinfection work, and the concentration of disinfectant should not be reduced at will for a long time.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R187
【参考文献】
相关期刊论文 前10条
1 范春,牛其昌,王颖;铜绿假单胞菌生物膜耐药性机制及防治[J];国外医药(抗生素分册);2004年05期
2 周庭权;黄文祥;贾蓓;;细菌对消毒剂抗性的研究进展[J];国外医药(抗生素分册);2008年01期
3 陈杨芳;邹珂;;聚维酮碘对铜绿假单胞菌杀菌效果及抗性的观察[J];全科医学临床与教育;2009年04期
4 党京丹;;医院内感染的发生和预防[J];临床和实验医学杂志;2009年10期
5 王正如;桂燕军;;湖北省黄梅县村卫生室2008年消毒效果分析[J];中国卫生监督杂志;2009年03期
6 陈昭斌,张朝武,叶梅君,殷强仲,余倩;使用中消毒剂分离CNS的抗消毒剂性研究[J];现代预防医学;1999年01期
7 孟甄,金建玲,刘玉庆,高培基;细菌耐药性的诱导与消除[J];中国药理学通报;2003年09期
8 罗涛;吴晓鸥;陈扬芳;张德亭;;体外诱导铜绿假单胞菌对亚胺培南和美罗培南耐药性的比较[J];中国药物与临床;2009年03期
9 支红梅;;院内感染的预防护理进展(综述)[J];中国城乡企业卫生;2008年02期
10 李长青;;西城区基层医疗单位消毒工作质量调查[J];中国消毒学杂志;2005年04期
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