解脲支原体在健康孕妇及新生儿泌尿生殖道中的分子流行病学研究
发布时间：2018-09-05 07:36
【摘要】：目的:研究解脲支原体(Ureaplasma urealyticum,Uu)在健康晚孕期孕妇以及健康新生儿泌尿生殖道中的检出情况与分群分型,以探讨Uu在该人群泌尿生殖道中的流行病学情况。 方法:收集健康晚孕期孕妇宫颈口分泌物85例,足月分娩的健康新生儿外阴分泌物124例,以上标本包括母婴37对。收集到的分泌物直接接种于解脲支原体液体选择性培养基进行初筛,初筛阳性的标本用PCR法进行分群。对Uu一群的标本再用PCR法及酶切法进一步分型。采用SPSS 10.0统计软件包进行统计学分析。 结果:85例健康晚孕期孕妇中Uu阳性51例(60.00%),其中单纯生物一群40例(78.43%),单纯生物二群7例(13.73%),两群混合4例(7.84%)。生物一群中1型11例(27.50%),6型10例(25.00%),3/14型14例(35.00%),混合型5例(12.50%)。124例新生儿中Uu阳性13例(10.48%),其中,经阴娩58例中阳性12例(20.69%),剖宫产66例中阳性1例(1.52%),两组阳性率差别有统计学意义(x~2=12.09,P0.01)。77例男婴中阳性4例(5.19%),47例女婴中阳性9例(19.15%),两组阳性率差别有统计学意义(x~2=6.055,P0.05)。13例Uu阳性的新生儿标本中,生物一群9例(69.23%),生物二群3例(23.08%),混合群1例(7.6996)。生物一群中1型2例,6型5例,3/14型1例,混合型1例。37对母婴中,母亲Uu阳性20例(其中经阴娩阳性14例,剖宫产阳性6例),婴儿阳性6例,均为经阴分娩婴儿,新生儿的Uu分型结果与其母亲Uu分型结果相一致。 结论:解脲支原体在健康晚孕期孕妇及足月分娩的健康新生儿泌尿生殖道中的携带率分别为60.00%与10.48%,经阴分娩组新生儿Uu的检出率高于剖宫产组,女婴外阴Uu检出率高于男婴,各人群组分离的Uu以生物一群为主;新生儿泌尿生殖道Uu分型结果与其母亲相一致,母亲的产道可能是新生儿泌尿生殖道Uu存在的最初来源。Uu可能属于人体的一种共生微生物。
[Abstract]:Objective: to study the detection and classification of Ureaplasma Urealyticum (Ureaplasma urealyticum,Uu) in the genitourinary tract of pregnant women and newborns during late pregnancy and to explore the epidemiology of Uu in urogenital tract. Methods: 85 cases of cervical secretion from pregnant women and 124 cases of vulva secretions from term delivery were collected, including 37 pairs of mothers and infants. The collected secretions were inoculated directly into Ureaplasma Urealyticum liquid selective medium for primary screening. The positive samples were divided into groups by PCR method. A group of Uu specimens were further typed by PCR and enzyme digestion. SPSS 10.0 statistical software package was used for statistical analysis. Results among 85 healthy pregnant women with late pregnancy, 51 (60.00%) were positive for Uu, including 40 (78.43%) in the first group, 7 (13.73%) in the second group, and 4 (7.84%) in the mixed group. There were 11 cases (27.50%) of type 1 in biological group, 10 cases (25.00%) of type 6, 14 cases of type 3 / 14 (35.00%), and 13 cases (10.48%) of positive Uu in 5 cases (12.50%) of mixed type. Of 58 cases of vaginal delivery, 12 cases (20.69%) were positive, and 66 cases of cesarean section were positive in 1 case (1.52%). There was a significant difference between the two groups (xan212.09 / P0.01) .77 male infants were positive in 4 cases (5.19%) and 47 cases in female infants (9 cases (19.15%). There was a significant difference between the two groups (XJ 26.055, P0.05). 13 cases of Uu. Positive neonatal specimens, There were 9 cases (69.23%) in the first group, 3 cases (23.08%) in the second group and 1 case (7.6996) in the mixed group. In one group of biology, 2 cases of type 1, 5 cases of type 6, 1 case of type 3 / 14, and 1 case of mixed type. 37 pairs of mother and infant, 20 cases of mother Uu positive (14 cases of transvaginal delivery, 6 cases of cesarean section), 6 cases of positive infant, all of them were transvaginal delivery. The results of Uu typing of newborns were consistent with the results of Uu typing of their mothers. Conclusion: the carrying rates of Ureaplasma Urealyticum in the genitourinary tract of healthy late pregnant women and full-term newborns were 60.00% and 10.48, respectively. The detection rate of Uu was higher in the transvaginal delivery group than in the cesarean section group, and the detection rate of vulvar Uu in female infants was higher than that in male infants. The Uu isolated from each group was mainly biotic, and the Uu typing results of neonate genitourinary tract were consistent with those of mother. The mother's birth canal might be the original source of neonatal urogenital tract Uu. Uu might belong to a symbiotic microorganism of human body.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R181.3
本文编号：2223615
[Abstract]:Objective: to study the detection and classification of Ureaplasma Urealyticum (Ureaplasma urealyticum,Uu) in the genitourinary tract of pregnant women and newborns during late pregnancy and to explore the epidemiology of Uu in urogenital tract. Methods: 85 cases of cervical secretion from pregnant women and 124 cases of vulva secretions from term delivery were collected, including 37 pairs of mothers and infants. The collected secretions were inoculated directly into Ureaplasma Urealyticum liquid selective medium for primary screening. The positive samples were divided into groups by PCR method. A group of Uu specimens were further typed by PCR and enzyme digestion. SPSS 10.0 statistical software package was used for statistical analysis. Results among 85 healthy pregnant women with late pregnancy, 51 (60.00%) were positive for Uu, including 40 (78.43%) in the first group, 7 (13.73%) in the second group, and 4 (7.84%) in the mixed group. There were 11 cases (27.50%) of type 1 in biological group, 10 cases (25.00%) of type 6, 14 cases of type 3 / 14 (35.00%), and 13 cases (10.48%) of positive Uu in 5 cases (12.50%) of mixed type. Of 58 cases of vaginal delivery, 12 cases (20.69%) were positive, and 66 cases of cesarean section were positive in 1 case (1.52%). There was a significant difference between the two groups (xan212.09 / P0.01) .77 male infants were positive in 4 cases (5.19%) and 47 cases in female infants (9 cases (19.15%). There was a significant difference between the two groups (XJ 26.055, P0.05). 13 cases of Uu. Positive neonatal specimens, There were 9 cases (69.23%) in the first group, 3 cases (23.08%) in the second group and 1 case (7.6996) in the mixed group. In one group of biology, 2 cases of type 1, 5 cases of type 6, 1 case of type 3 / 14, and 1 case of mixed type. 37 pairs of mother and infant, 20 cases of mother Uu positive (14 cases of transvaginal delivery, 6 cases of cesarean section), 6 cases of positive infant, all of them were transvaginal delivery. The results of Uu typing of newborns were consistent with the results of Uu typing of their mothers. Conclusion: the carrying rates of Ureaplasma Urealyticum in the genitourinary tract of healthy late pregnant women and full-term newborns were 60.00% and 10.48, respectively. The detection rate of Uu was higher in the transvaginal delivery group than in the cesarean section group, and the detection rate of vulvar Uu in female infants was higher than that in male infants. The Uu isolated from each group was mainly biotic, and the Uu typing results of neonate genitourinary tract were consistent with those of mother. The mother's birth canal might be the original source of neonatal urogenital tract Uu. Uu might belong to a symbiotic microorganism of human body.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R181.3
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